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SOCS-1 Antibody (YA5407)

(Synonyms: Suppressor of cytokine signaling 1; SOCS-1; JAK-binding protein; JAB; STAT-induced STAT inhibitor 1; SSI-1; Tec-interacting protein 3; TIP-3; )
Customer Review

Based on 1 Customer Validation

SOCS-1 Antibody (YA5407) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to SOCS-1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    IHC-P, WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SOCS-1 Antibody (YA5407)
  • Experimental Validation Results for SOCS-1 Antibody (YA5407)
  • SOCS-1 Antibody (YA5407) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
Dilution Ratio 1:50-200 1:1000-2000

Product Details

Description

SOCS-1 Antibody (YA5407) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to SOCS-1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Immunogen

Synthesized peptide derived from human SOCS-1

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Unmodified

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SOCS-1 Antibody (YA5407)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using SOCS-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-1 Antibody (YA5407)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using SOCS-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    SOCS-1 is essential negative regulator of type I and type II interferon (IFN) signaling, as well as that of other cytokines, including IL2, IL4, IL6 and leukemia inhibitory factor (LIF). Downregulates cytokine signaling by inhibiting the JAK/STAT signaling pathway. Acts by binding to JAK proteins and to IFNGR1 and inhibiting their kinase activity. In vitro, suppresses Tec protein-tyrosine activity. Regulates IFN-gamma (IFNG)-mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins[1][2][3][4][5].

  • Subcellular Localization

    Nucleus; Cytoplasmic vesicle

  • Expression


    Tissue_specificity:It is expressed in all tissues, with high expression in the spleen, small intestine, and peripheral blood leukocytes.

    Induction:By a subset of cytokines including those belonging to the interferon, interleukin and colony-stimulating factor families

  • Subunit

    Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV.

  • SwissProt ID

    O15524

  • Gene ID
    8651 [NCBI]
  • Synonyms

    Suppressor of cytokine signaling 1; SOCS-1; JAK-binding protein; JAB; STAT-induced STAT inhibitor 1; SSI-1; Tec-interacting protein 3; TIP-3;

SOCS-1 Antibody (YA5407) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using SOCS-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using SOCS-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SOCS-1 Antibody (YA5407) Product Image
    1/16
  • Experimental Validation Results for SOCS-1 Antibody (YA5407)
  • Experimental Validation Results for SOCS-1 Antibody (YA5407)
  • SOCS-1 Antibody (YA5407) Product Image
  • Experimental Validation Results for SOCS-1 Antibody (YA5407)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using SOCS-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-1 Antibody (YA5407)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using SOCS-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SOCS-1 Antibody (YA5407)
  • Experimental Validation Results for SOCS-1 Antibody (YA5407)
  • SOCS-1 Antibody (YA5407) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using SOCS-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SOCS-1 Antibody (YA5407) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using SOCS-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SOCS-1 Antibody (YA5407) Product Image IHC-P
  • SOCS-1 Antibody (YA5407) Product Image IHC-P