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Endothelin Receptor B Antibody (YA3255)

(Synonyms: Endothelin B receptor; ET-B; ET-BR; Endothelin receptor non-selective type)
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Based on 1 Customer Validation

Endothelin Receptor B Antibody (YA3255) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Endothelin Receptor B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Endothelin Receptor B Antibody (YA3255)
  • Endothelin Receptor B Antibody (YA3255) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:50

Product Details

Description

Endothelin Receptor B Antibody (YA3255) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Endothelin Receptor B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Species Reactivity Database

Entrez Gene: 1910 Human

SwissProt: P24530 Human

Immunogen

A synthesized peptide derived from human Endothelin B Receptor aa35-90/442.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Endothelin Receptor B Antibody (YA3255)
    Western blot analysis was performed on protein extracts (40 μg) from HeLa (lane 2), Caco-2 (lane 3), SH-SY5Y (lane 4), and DU145 (lane 5) using Endothelin Receptor B antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Endothelin Receptor B is a Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells

  • Isoforms & Post-Translational Modification

    P24530 has 3 isomers: P24530-1: 49644 Da (predicted); P24530-2: 48710 Da (predicted); P24530-3: 59480 Da (predicted).
    Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405

  • SwissProt ID

    P24530

  • Gene ID
    1910 [NCBI]
  • Synonyms

    Endothelin B receptor; ET-B; ET-BR; Endothelin receptor non-selective type

  • Research Field

    Signal Transduction

Endothelin Receptor B Antibody (YA3255) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (40 μg) from HeLa (lane 2), Caco-2 (lane 3), SH-SY5Y (lane 4), and DU145 (lane 5) using Endothelin Receptor B antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Endothelin Receptor B Antibody (YA3255) Product Image
    1/16
  • Experimental Validation Results for Endothelin Receptor B Antibody (YA3255)
  • Endothelin Receptor B Antibody (YA3255) Product Image
  • Experimental Validation Results for Endothelin Receptor B Antibody (YA3255)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (40 μg) from HeLa (lane 2), Caco-2 (lane 3), SH-SY5Y (lane 4), and DU145 (lane 5) using Endothelin Receptor B antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Endothelin Receptor B Antibody (YA3255)
  • Endothelin Receptor B Antibody (YA3255) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (40 μg) from HeLa (lane 2), Caco-2 (lane 3), SH-SY5Y (lane 4), and DU145 (lane 5) using Endothelin Receptor B antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Endothelin Receptor B Antibody (YA3255) Product Image WB