Phospho-mTOR (Ser2481) Antibody (YA7614)
(Synonyms: FRAP, FRAP1, FRAP2, RAFT1, RAPT1, MTOR, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, Tyrosine-protein kinase mTOR, mTOR)
Based on 1 Customer Validation
Phospho-mTOR (Ser2481) Antibody (YA7614) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-mTOR (Ser2481).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 |
Product Details
Phospho-mTOR (Ser2481) Antibody (YA7614) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-mTOR (Ser2481).
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 289 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 289 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
mTOR (mammalian target of Rapamycin) is a protein that in humans is encoded by the mTOR gene. mTOR is a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. mTOR belongs to the phosphatidylinositol 3-kinase-related kinase protein family. mTOR integrates the input from upstream pathways, including growth factors and amino acids. mTOR also senses cellular nutrient, oxygen, and energy levels. The mTOR pathway is dysregulated in human diseases, such as diabetes, obesity, depression, and certain cancers. Rapamycin inhibits mTOR by associating with its intracellular receptor FKBP12. The FKBP12-rapamycin complex binds directly to the FKBP12-Rapamycin Binding (FRB) domain of mTOR, inhibiting its activity.
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Subcellular Localization
Lysosome membrane,Endoplasmic reticulum membrane,Golgi apparatus membrane,Cell membrane,Mitochondrion outer membrane,Cytoplasm,Nucleus,Nucleus, PML body,Microsome membrane,Cytoplasmic vesicle, phagosome
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Expression
Tissue_Specificity: Expressed in numerous tissues, with highest levels in testis -
Isoforms & Post-Translational Modification
P42345: 2549 amino acids, molecular weight 288892 Da.
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Subunit
Part of the mechanistic target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8 and RPTOR (PubMed:12150925, PubMed:12150926, PubMed:12408816, PubMed:12718876, PubMed:18925875, PubMed:20542007, PubMed:23636326, PubMed:24403073, PubMed:26678875, PubMed:27909983, PubMed:29236692, PubMed:31601764, PubMed:34519268, PubMed:34519269, PubMed:36697823)
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SwissProt ID
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Synonyms
FRAP, FRAP1, FRAP2, RAFT1, RAPT1, MTOR, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, Tyrosine-protein kinase mTOR, mTOR
Documentation
References
[1]. Laplante M, et al. mTOR signaling in growth control and disease. Cell. 2012 Apr 13;149(2):274-93. [Content Brief]
[2]. Panwar V, et al. Multifaceted role of mTOR (mammalian target of rapamycin) signaling pathway in human health and disease. Signal Transduct Target Ther. 2023 Oct 2;8(1):375. [Content Brief]
[3]. Yong J, et al. Regulation of transcriptome plasticity by mTOR signaling pathway. Exp Mol Med. 2025 Aug;57(8):1623-1630. [Content Brief]
[4]. Ballesteros-Álvarez J, et al. mTORC2: The other mTOR in autophagy regulation. Aging Cell. 2021 Aug;20(8):e13431. [Content Brief]
[5]. Patel CH, et al. More TOR: The expanding role of mTOR in regulating immune responses. Immunity. 2025 Jul 8;58(7):1629-1645. [Content Brief]
[6]. Dai DF, et al. The mTOR signaling pathway in cardiac aging. J Cardiovasc Aging. 2023;3(3):24. [Content Brief]
[7]. Wang F, et al. Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function. Nat Commun. 2018 Nov 19;9(1):4874. [Content Brief]
[8]. Schaub T, et al. mTORC1 and mTORC2 Differentially Regulate Cell Fate Programs to Coordinate Osteoblastic Differentiation in Mesenchymal Stromal Cells. Sci Rep. 2019 Dec 27;9(1):20071. [Content Brief]
[9]. Mao B, et al. Overview of Research into mTOR Inhibitors. Molecules. 2022 Aug 19;27(16):5295. [Content Brief]
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