RbBP5 Antibody (YA5309)

Cat. No.: HY-P85617: Data Sheet User Guide for Antibodies
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RbBP5 Antibody (YA5309) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to RbBP5.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RbBP5 Antibody (YA5309) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to RbBP5.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q15291

Gene ID

5929 [NCBI]

Immunogen

Purified recombinant human RBBP5 protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Solution

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid (By similarity). Does not affect ES cell self-renewal (By similarity). Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters (PubMed:19131338). As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3 (PubMed:19556245). Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:19556245). In association with ASH2L and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed:21220120, PubMed:22266653)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:general expression

Isoforms & Post-Translational Modification：Q15291 has 2 isomers: Q15291-1: 59153 Da (predicted); Q15291-2: 55072 Da (predicted).

Subunit：Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30 (PubMed:16253997, PubMed:17355966, PubMed:17998332, PubMed:18838538).

Synonyms

RBBP 5; RBBP-5; RBBP5; RBBP5_HUMAN; RBQ 3; RBQ3; Retinoblastoma binding protein 5; Retinoblastoma binding protein RBQ3; Retinoblastoma binding protein5; Retinoblastoma-binding protein 5; Retinoblastoma-binding protein RBQ-3; SWD1; SWD1, Set1c WD40 repeat protein, homolog.

Documentation

User Guide for Antibodies (1077 KB)