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CD105 Antibody (YA7359)

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Based on 1 Customer Validation

CD105 Antibody (YA7359) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to CD105.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG,Kappa

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD105 Antibody (YA7359)
  • CD105 Antibody (YA7359) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:100-200 1:1000-5000 1:200-1000 1:5000-20000 1:50-200

Product Details

Description

CD105 Antibody (YA7359) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to CD105.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 95 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 70 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD105 Antibody (YA7359)
    Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2), HeLa (lane 3), HT-1080 (lane 4), THP-1 (lane 5), HepG2 (lane 6), and SH-SY5Y (lane 7) using IREB2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    CD105 (Endoglin, ENG) is a TGF-β receptor accessory protein primarily expressed on mesenchymal stem cells and activated endothelial cells; it serves as a classic positive marker for the identification of mesenchymal stem cells (MSCs) and is a key target in research concerning angiogenesis, tumor neovascularization, and fibrosis.

  • Subcellular Localization

    Cell membrane

  • Expression


    Tissue_specificity:Detected in umbilical cord membrane endothelial cells (PubMed: 10625079) . Detected in the placenta (protein level) (PubMed: 1692830) . Detected in endothelial cells (PubMed: 1692830) .

  • Isoforms & Post-Translational Modification

    CD105 has 2 isomers: P17813-1: 70578 Da (predicted); P17813-2: 67542 Da (predicted).

  • Subunit

    Homodimer; disulfide-linked. Forms a heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGFB1 and TGFB2 with high affinity, but not TGFB3. Interacts with GDF2, forming a heterotetramer with a 2:2 stoichiometry. Interacts with ACVRL1. Can form a heteromeric complex with GDF2 and ACVRL1. Interacts with BMP10. Interacts with DYNLT4. Interacts with ARRB2

  • SwissProt ID

    P17813

CD105 Antibody (YA7359) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2), HeLa (lane 3), HT-1080 (lane 4), THP-1 (lane 5), HepG2 (lane 6), and SH-SY5Y (lane 7) using IREB2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CD105 Antibody (YA7359) Product Image
    1/16
  • Experimental Validation Results for CD105 Antibody (YA7359)
  • CD105 Antibody (YA7359) Product Image
  • Experimental Validation Results for CD105 Antibody (YA7359)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2), HeLa (lane 3), HT-1080 (lane 4), THP-1 (lane 5), HepG2 (lane 6), and SH-SY5Y (lane 7) using IREB2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for CD105 Antibody (YA7359)
  • CD105 Antibody (YA7359) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2), HeLa (lane 3), HT-1080 (lane 4), THP-1 (lane 5), HepG2 (lane 6), and SH-SY5Y (lane 7) using IREB2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CD105 Antibody (YA7359) Product Image WB