GluR-2 Antibody (YA7446)

Cat. No.: HY-P87761: Data Sheet User Guide for Antibodies
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GluR-2 Antibody (YA7446) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to GluR-2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

GluR-2 Antibody (YA7446) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to GluR-2.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 99 kDa;

Observed band size: 99 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P42262

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
WB WB: Western Blot	1:10000-1:50000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Sensitivity	Endogenous	Purity	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG,Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed:20614889, PubMed:31300657, PubMed:8003671). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (PubMed:14687553). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium (PubMed:20614889, PubMed:8003671). The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (By similarity). Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity)

Subcellular Localization：Cell membrane,Postsynaptic cell membrane,Postsynaptic density membrane

Isoforms & Post-Translational Modification：P42262 has four isomers: P42262-1: 98821 Da (predicted); P42262-2: 98878 Da (predicted); P42262-3: 100594 Da (predicted); P42262-4: 93775 Da (predicted).
Palmitoylated丨Ubiquitinated by RNF167, leading to its degradation丨Phosphorylation at Tyr-876 is required for interaction with IQSEC1 and ARF6 activation, which in turn triggers AMPAR internalization for persistent synaptic depression丨N-glycosylated

Subunit：Homotetramer or heterotetramer of pore-forming glutamate receptor subunits (PubMed:14687553, PubMed:19651138, PubMed:20614889, PubMed:21531559, PubMed:8003671)

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/huh-7-cell.html [Content Brief]