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YB1 Antibody (YA004)

(Synonyms: NSEP1, YB1, YBX1, Y-box-binding protein 1, YB-1, CCAAT-binding transcription factor I subunit A, DNA-binding protein B, Enhancer factor I subunit A, Nuclease-sensitive element-binding protein 1, Y-box transcription factor, CBF-A, DBPB, EFI-A)
Customer Review

Based on 1 Customer Validation

YB1 Antibody (YA004) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to YB1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for YB1 Antibody (YA004)
  • Experimental Validation Results for YB1 Antibody (YA004)
  • Experimental Validation Results for YB1 Antibody (YA004)
  • YB1 Antibody (YA004) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:100-1:500 1:50-1:200 1:50-1:100 Use at an assay dependent concentration.

Product Details

Description

YB1 Antibody (YA004) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to YB1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human YB1.AA range:275-324.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102464

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for YB1 Antibody (YA004)
    Western blot analysis of extracts from Hela (lane 1) and 293T (lane 2) and RAW264.7 (lane 3) using YB1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for YB1 Antibody (YA004)
    Immunocytochemistry analysis of Hela cells labeling YB1 with YB1 Antibody (HY-P80375) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with YB1 Antibody (HY-P80375) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for YB1 Antibody (YA004)
    Immunocytochemistry analysis of Hela cells labeling YB1 with YB1 Antibody (HY-P80375) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with YB1 Antibody (HY-P80375) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    YB-1 (Y box binding protein 1) is a multifunctional transcription and translation factor, and is over-expressed in various human cancers. YB-1 is a 36 kDa protein, and consists of three domains: the alanine/proline-rich N-terminal domain (A/P domain)， the cold shock domain (CSD)， and the large C terminal domain (CTD) with alternating clusters of positively and negatively charged amino acid residues. YB-1 regulates cancer cell proliferation and progression, cell survival, cell proliferation and differentiation, stress response, and malignant cell transformation. YB-1 also participates in a wide variety of DNA/RNA-dependent events, including DNA reparation, pre-mRNA transcription and splicing, mRNA packaging, and regulation of mRNA stability and translation[1][2].

  • Subcellular Localization

    Cytoplasm; Nucleus; Cytoplasmic granule; Secreted; Secreted, extracellular exosome; Cytoplasm, P-body

  • Subunit

    Homodimer in the presence of ATP (PubMed:10817758, PubMed:11851341). Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1 (PubMed:19029303).

  • SwissProt ID

    P67809

  • Gene ID
    4904 [NCBI]
  • Synonyms

    NSEP1, YB1, YBX1, Y-box-binding protein 1, YB-1, CCAAT-binding transcription factor I subunit A, DNA-binding protein B, Enhancer factor I subunit A, Nuclease-sensitive element-binding protein 1, Y-box transcription factor, CBF-A, DBPB, EFI-A

  • Research Field

    Tags & Cell Markers

[1]. Maurya PK, et al. Role of Y Box Protein-1 in cancer: As potential biomarker and novel therapeutic target. J Cancer. 2017 Jul 3;8(10):1900-1907. [Content Brief]

[2]. Lyabin DN, et al. YB-1 protein: functions and regulation. Wiley Interdiscip Rev RNA. 2014 Jan-Feb;5(1):95-110. [Content Brief]

YB1 Antibody (YA004) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 1) and 293T (lane 2) and RAW264.7 (lane 3) using YB1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling YB1 with YB1 Antibody (HY-P80375) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with YB1 Antibody (HY-P80375) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling YB1 with YB1 Antibody (HY-P80375) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with YB1 Antibody (HY-P80375) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • YB1 Antibody (YA004) Product Image
    1/16
  • Experimental Validation Results for YB1 Antibody (YA004)
  • Experimental Validation Results for YB1 Antibody (YA004)
  • Experimental Validation Results for YB1 Antibody (YA004)
  • YB1 Antibody (YA004) Product Image
  • Experimental Validation Results for YB1 Antibody (YA004)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 1) and 293T (lane 2) and RAW264.7 (lane 3) using YB1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for YB1 Antibody (YA004)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling YB1 with YB1 Antibody (HY-P80375) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with YB1 Antibody (HY-P80375) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for YB1 Antibody (YA004)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling YB1 with YB1 Antibody (HY-P80375) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with YB1 Antibody (HY-P80375) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for YB1 Antibody (YA004)
  • Experimental Validation Results for YB1 Antibody (YA004)
  • Experimental Validation Results for YB1 Antibody (YA004)
  • YB1 Antibody (YA004) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 1) and 293T (lane 2) and RAW264.7 (lane 3) using YB1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • YB1 Antibody (YA004) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling YB1 with YB1 Antibody (HY-P80375) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with YB1 Antibody (HY-P80375) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • YB1 Antibody (YA004) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling YB1 with YB1 Antibody (HY-P80375) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with YB1 Antibody (HY-P80375) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • YB1 Antibody (YA004) Product Image WB
  • YB1 Antibody (YA004) Product Image ICC
  • YB1 Antibody (YA004) Product Image ICC