Importin 9/RANBP9 Antibody (YA1625)(PBS only)

Cat. No.: HY-P81880A: Data Sheet User Guide for Antibodies
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Importin 9/RANBP9 Antibody (YA1625) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Importin 9/RANBP9.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Importin 9/RANBP9 Antibody (YA1625) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Importin 9/RANBP9.

Host

Rabbit

Molecular Weight

Predicted band size: 116 kDa;

Observed band size: 116 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q96P70

Gene ID

55705 [NCBI]

Immunogen

A synthetic peptide of human Importin 9

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Nuclear transport receptor that mediates nuclear import of proteins, such as histones, proteasome and actin (PubMed:11823430, PubMed:30855230, PubMed:34711951). Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:11823430). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11823430). At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran (PubMed:11823430). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11823430). Mediates the import of pre-assembled proteasomes into the nucleus; AKIRIN2 acts as a molecular bridge between IPO9 and the proteasome complex (PubMed:11823430, PubMed:34711951). Mediates the nuclear import of histones H2A, H2B, H4 and H4 (PubMed:11823430, PubMed:30855230). In addition to nuclear import, also acts as a chaperone for histones by preventing inappropriate non-nucleosomal interactions (PubMed:30855230). Mediates the nuclear import of actin (By similarity)

Subcellular Localization：Cytoplasm; Nucleus

Subunit：Interacts with histones H2A, H2B, H3 and H4 (PubMed:11823430, PubMed:30855230). The binding is coupled to RanGTP cycles (PubMed:11823430). Interacts with AKIRIN2; promoting association with pre-assembled proteasomes (PubMed:34711951). Associates with pre-assembled proteasomes; interaction is indirect and mediated via interaction with AKIRIN2 (PubMed:34711951). Interacts with PPP2R1A and PPP2R1B (PubMed:12670497)

Research Field

Signal Transduction

Synonyms

importin 9; Imp9; Ran-binding protein 9; RanBP9

Documentation

User Guide for Antibodies (1077 KB)