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NPY5R Antibody (YA6894)

(Synonyms: Neuropeptide Y receptor type 5 antibody)
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Based on 1 Customer Validation

NPY5R Antibody (YA6894) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NPY5R.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NPY5R Antibody (YA6894)
  • NPY5R Antibody (YA6894) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:2000 1:50-1:200 1:50-1:200 1:500-1:1000

Product Details

Description

NPY5R Antibody (YA6894) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NPY5R.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 51 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size：51 kDa
Immunogen

Synthetic peptide within human NPY5R aa 1-50/445.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NPY5R Antibody (YA6894)
    Western blot analysis of extracts from unboiled HeLa (lane 1(20μg)) 、unboiled HepG2 (lane 2(20μg)) 、unboiled Mouse brain (lane 3(40μg)) and unboiled Rat brain (lane 4(40μg)) using NPY5R Antibody (HY-P87206) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    NPY5R receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders

  • Subcellular Localization

    Cell membrane

  • Expression


    Tissue_Specificity: Brain; hypothalamus

  • Isoforms & Post-Translational Modification

    Q15761: 445 amino acids, molecular weight 50727 Da.

  • SwissProt ID

    Q15761

  • Synonyms

    Neuropeptide Y receptor type 5 antibody

NPY5R Antibody (YA6894) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from unboiled HeLa (lane 1(20μg)) 、unboiled HepG2 (lane 2(20μg)) 、unboiled Mouse brain (lane 3(40μg)) and unboiled Rat brain (lane 4(40μg)) using NPY5R Antibody (HY-P87206) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • NPY5R Antibody (YA6894) Product Image
    1/16
  • Experimental Validation Results for NPY5R Antibody (YA6894)
  • NPY5R Antibody (YA6894) Product Image
  • Experimental Validation Results for NPY5R Antibody (YA6894)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from unboiled HeLa (lane 1(20μg)) 、unboiled HepG2 (lane 2(20μg)) 、unboiled Mouse brain (lane 3(40μg)) and unboiled Rat brain (lane 4(40μg)) using NPY5R Antibody (HY-P87206) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NPY5R Antibody (YA6894)
  • NPY5R Antibody (YA6894) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from unboiled HeLa (lane 1(20μg)) 、unboiled HepG2 (lane 2(20μg)) 、unboiled Mouse brain (lane 3(40μg)) and unboiled Rat brain (lane 4(40μg)) using NPY5R Antibody (HY-P87206) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • NPY5R Antibody (YA6894) Product Image WB