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MTAP Antibody (YA3085)

(Synonyms: BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249)
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Based on 1 Customer Validation

MTAP Antibody (YA3085) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MTAP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MTAP Antibody (YA3085)
  • MTAP Antibody (YA3085) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

MTAP Antibody (YA3085) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MTAP.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 31 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human MTAP

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105453

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MTAP Antibody (YA3085)
    Western blot analysis of extracts from NIH/3T3(lane 2(20ug)，A549(lane 3(20ug) and MCF-7(lane 4(20ug) using MTAP Antibody (HY-P83340) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    MTAP catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:Ubiquitously expressed

  • Isoforms & Post-Translational Modification

    Q13126 has 7 isomers: Q13126-1: 31236 Da (predicted); Q13126-2: 38356 Da (predicted); Q13126-3: 36936 Da (predicted); Q13126-4: 30838 Da (predicted); Q13126-5: 33797 Da (predicted); Q13126-6: 32376 Da (predicted); Q13126-7: 26278 Da (predicted).

  • Subunit

    Homotrimer

  • SwissProt ID

    Q13126

  • Gene ID
    4507 [NCBI]
  • Synonyms

    BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249

  • Research Field

    Signal Transduction

MTAP Antibody (YA3085) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from NIH/3T3(lane 2(20ug)，A549(lane 3(20ug) and MCF-7(lane 4(20ug) using MTAP Antibody (HY-P83340) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MTAP Antibody (YA3085) Product Image
    1/16
  • Experimental Validation Results for MTAP Antibody (YA3085)
  • MTAP Antibody (YA3085) Product Image
  • Experimental Validation Results for MTAP Antibody (YA3085)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from NIH/3T3(lane 2(20ug)，A549(lane 3(20ug) and MCF-7(lane 4(20ug) using MTAP Antibody (HY-P83340) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MTAP Antibody (YA3085)
  • MTAP Antibody (YA3085) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from NIH/3T3(lane 2(20ug)，A549(lane 3(20ug) and MCF-7(lane 4(20ug) using MTAP Antibody (HY-P83340) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MTAP Antibody (YA3085) Product Image WB