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  4. Monoclonal Antibodies Recombinant Antibodies
  5. MTAP Antibody (YA3085)

MTAP Antibody (YA3085) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MTAP.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • 제품 설명

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제품 설명

MTAP Antibody (YA3085) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MTAP.
Host

Rabbit
Clonality

Recombinant, Monoclonal
분자량
Predicted band size: 31 kDa;
Observed band size: 31 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human MTAP
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from NIH/3T3(lane 2(20ug)，A549(lane 3(20ug) and MCF-7(lane 4(20ug) using MTAP Antibody (HY-P83340) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates
Subcellular Localization：Cytoplasm; Nucleus
Expression：
Tissue_specificity:Ubiquitously expressed
Isoforms & Post-Translational Modification：Q13126 has 7 isomers: Q13126-1: 31236 Da (predicted); Q13126-2: 38356 Da (predicted); Q13126-3: 36936 Da (predicted); Q13126-4: 30838 Da (predicted); Q13126-5: 33797 Da (predicted); Q13126-6: 32376 Da (predicted); Q13126-7: 26278 Da (predicted).
Subunit：Homotrimer
RRID
Database

Entrez Gene: 4507 Human ; 66902 Mouse ;

SwissProt: Q13126 Human ; Q9CQ65 Mouse ;

OMIM: 112250 Human

Research Field

Signal Transduction
Synonyms
BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249
각종 서류
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)
References

MTAP Antibody (YA3085) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
MTAP Antibody (YA3085)
Cat. No.:
HY-P83340
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SAFETY DATA SHEET (SDS)

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