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IL-32 Antibody (YA2911)

(Synonyms: IL-32; IL 32alpha; IL 32beta; IL 32delta; IL 32gamma; IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd)
Customer Review

Based on 1 Customer Validation

IL-32 Antibody (YA2911) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-32.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for IL-32 Antibody (YA2911)
  • IL-32 Antibody (YA2911) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

IL-32 Antibody (YA2911) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-32.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Species Reactivity Database

Entrez Gene: 9235 Human

SwissProt: P24001 Human

Immunogen

A synthesized peptide derived from human IL32 aa200-234.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105281

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IL-32 Antibody (YA2911)
    Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using IL-32 Antibody (HY-P83166) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    IL-32 is a Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Selectively expressed in lymphocytes. Expression is more prominent in immune cells than in non-immune cells

    Induction:Expression increased after activation of T-cells by mitogens or activation of NK cells by IL2/interleukin-2

  • Isoforms & Post-Translational Modification

    P24001 has 7 isomers: P24001-1: 26676 Da (predicted); P24001-2: 21737 Da (predicted); P24001-3: 20600 Da (predicted); P24001-4: 14909 Da (predicted); P24001-5: 19389 Da (predicted); P24001-6: 20777 Da (predicted); P24001-7: 17003 Da (predicted).

  • SwissProt ID

    P24001

  • Gene ID
    9235 [NCBI]
  • Synonyms

    IL-32; IL 32alpha; IL 32beta; IL 32delta; IL 32gamma; IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd

  • Research Field

    Immunology

IL-32 Antibody (YA2911) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using IL-32 Antibody (HY-P83166) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • IL-32 Antibody (YA2911) Product Image
    1/16
  • Experimental Validation Results for IL-32 Antibody (YA2911)
  • IL-32 Antibody (YA2911) Product Image
  • Experimental Validation Results for IL-32 Antibody (YA2911)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using IL-32 Antibody (HY-P83166) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IL-32 Antibody (YA2911)
  • IL-32 Antibody (YA2911) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using IL-32 Antibody (HY-P83166) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • IL-32 Antibody (YA2911) Product Image WB