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  4. IL-32 Antibody (YA2911)

IL-32 Antibody (YA2911) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-32.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

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  • Documentation

Description

IL-32 Antibody (YA2911) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-32.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight
Predicted band size: 27 kDa;
Observed band size: 23 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human IL32 aa200-234.

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid

Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image
WB
  • Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using IL-32 Antibody (HY-P83166) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function:Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK
Subcellular Localization:Secreted
Expression:
Tissue_specificity:Selectively expressed in lymphocytes. Expression is more prominent in immune cells than in non-immune cells

Induction:Expression increased after activation of T-cells by mitogens or activation of NK cells by IL2/interleukin-2
Isoforms & Post-Translational Modification:P24001 has 7 isomers: P24001-1: 26676 Da (predicted); P24001-2: 21737 Da (predicted); P24001-3: 20600 Da (predicted); P24001-4: 14909 Da (predicted); P24001-5: 19389 Da (predicted); P24001-6: 20777 Da (predicted); P24001-7: 17003 Da (predicted).
RRID
Database
Research Field

Immunology

Synonyms
IL-32; IL 32alpha; IL 32beta; IL 32delta; IL 32gamma; IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd
Documentation

IL-32 Antibody (YA2911) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
IL-32 Antibody (YA2911)
Cat. No.:
HY-P83166
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