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Asparagine Synthetase Antibody (YA608)

(Synonyms: TS11; ASNSD; ASNS; Asparagine synthetase [glutamine-hydrolyzing])
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Based on 1 Customer Validation

Asparagine Synthetase Antibody (YA608) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Asparagine Synthetase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, mIHC

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Asparagine Synthetase Antibody (YA608)
  • Asparagine Synthetase Antibody (YA608) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
mIHC Info
mIHC: Multiplex Immunohistochemical
Dilution Ratio 1:500-1000 1:50-100 1:100-500

Product Details

Description

Asparagine Synthetase Antibody (YA608) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Asparagine Synthetase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 64 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 64 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Asparagine synthetase.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102909

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Asparagine Synthetase Antibody (YA608)
    Western blot analysis was performed on extracts from K562 (lane 1, 15 μg), 293 (lane 2, 15 μg), A431 (lane 3, 15 μg), Rat pancreas (lane 4, 15 μg), Hela (lane 5, 15 μg), and 4T1 (lane 6, 15 μg) using Asparagine Synthetase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Nutrient bioavailability in the tumor microenvironment plays a pivotal role in tumor proliferation and metastasis. Among these nutrients, glutamine is a key substance that promotes tumor growth and proliferation, and its downstream metabolite Asparagine is also crucial in tumors. Studies have shown that when glutamine is exhausted, tumor cells can rely on Asparagine to sustain their growth. Given the reliance of tumor cell proliferation on Asparagine, restricting its bioavailability has emerged as promising strategy in cancer treatment. For instance, the use of asparaginase, an enzyme that depletes Asparagine, has been one of the key chemotherapies for acute lymphoblastic leukemia (ALL)[1].

  • Isoforms & Post-Translational Modification

    Asparagine has 3 isomers: P08243-1: 64370 Da (predicted); P08243-2: 62168 Da (predicted); P08243-3: 54818 Da (predicted).

  • SwissProt ID

    P08243

  • Gene ID
    440 [NCBI]
  • Synonyms

    TS11; ASNSD; ASNS; Asparagine synthetase [glutamine-hydrolyzing]

  • Research Field

    Signal Transduction

References

[1]. Wang X, et al. Asparagine: A key metabolic junction in targeted tumor therapy. Pharmacol Res. 2024 Aug;206:107292.

Asparagine Synthetase Antibody (YA608) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from K562 (lane 1, 15 μg), 293 (lane 2, 15 μg), A431 (lane 3, 15 μg), Rat pancreas (lane 4, 15 μg), Hela (lane 5, 15 μg), and 4T1 (lane 6, 15 μg) using Asparagine Synthetase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Asparagine Synthetase Antibody (YA608) Product Image
    1/16
  • Experimental Validation Results for Asparagine Synthetase Antibody (YA608)
  • Asparagine Synthetase Antibody (YA608) Product Image
  • Experimental Validation Results for Asparagine Synthetase Antibody (YA608)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from K562 (lane 1, 15 μg), 293 (lane 2, 15 μg), A431 (lane 3, 15 μg), Rat pancreas (lane 4, 15 μg), Hela (lane 5, 15 μg), and 4T1 (lane 6, 15 μg) using Asparagine Synthetase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Asparagine Synthetase Antibody (YA608)
  • Asparagine Synthetase Antibody (YA608) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from K562 (lane 1, 15 μg), 293 (lane 2, 15 μg), A431 (lane 3, 15 μg), Rat pancreas (lane 4, 15 μg), Hela (lane 5, 15 μg), and 4T1 (lane 6, 15 μg) using Asparagine Synthetase Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Asparagine Synthetase Antibody (YA608) Product Image WB