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RPL18A Antibody (YA4170)

(Synonyms: L18A)
Customer Review

Based on 1 Customer Validation

RPL18A Antibody (YA4170) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RPL18A.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RPL18A Antibody (YA4170)
  • Experimental Validation Results for RPL18A Antibody (YA4170)
  • RPL18A Antibody (YA4170) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000

Product Details

Description

RPL18A Antibody (YA4170) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RPL18A.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Immunogen

Purified recombinant fragment of human RPL18A (AA: 50-176) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RPL18A Antibody (YA4170)
    Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 1), SH-SY5Y (lane 2), HeLa (lane 3), HepG2 (lane 4), A549 (lane 5), and Caco-2 (lane 6) using RPL18A antibody. Proteins were transferred onto a 0.45 μm NC membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody β-actin (HY-P80438) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane was incubated with HRP-conjugated goat anti-mouse IgG (H&L) secondary antibody (HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for RPL18A Antibody (YA4170)
    Western blot analysis of extracts from SH-SY5Y (lane2, 40μg), HepG2 (lane3, 40μg), HEK293 (lane4, 20μg), MOLT-4 (lane5, 20μg) and Raji (lane6, 40μg) using RPL18A Antibody (HY-P84473). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 2 hour at room temperature.

Background

  • Function

    RPL18A component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell

  • Subcellular Localization

    Cytoplasm

  • Subunit

    Component of the large ribosomal subunit (PubMed:23636399, PubMed:32669547). Binds IPO9 with high affinity (PubMed:11823430)

  • SwissProt ID

    Q02543

  • Gene ID
    6142 [NCBI]
  • Synonyms

    L18A

RPL18A Antibody (YA4170) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 1), SH-SY5Y (lane 2), HeLa (lane 3), HepG2 (lane 4), A549 (lane 5), and Caco-2 (lane 6) using RPL18A antibody. Proteins were transferred onto a 0.45 μm NC membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody β-actin (HY-P80438) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane was incubated with HRP-conjugated goat anti-mouse IgG (H&L) secondary antibody (HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y (lane2, 40μg), HepG2 (lane3, 40μg), HEK293 (lane4, 20μg), MOLT-4 (lane5, 20μg) and Raji (lane6, 40μg) using RPL18A Antibody (HY-P84473). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 2 hour at room temperature.

  • RPL18A Antibody (YA4170) Product Image
    1/16
  • Experimental Validation Results for RPL18A Antibody (YA4170)
  • Experimental Validation Results for RPL18A Antibody (YA4170)
  • RPL18A Antibody (YA4170) Product Image
  • Experimental Validation Results for RPL18A Antibody (YA4170)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 1), SH-SY5Y (lane 2), HeLa (lane 3), HepG2 (lane 4), A549 (lane 5), and Caco-2 (lane 6) using RPL18A antibody. Proteins were transferred onto a 0.45 μm NC membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody β-actin (HY-P80438) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane was incubated with HRP-conjugated goat anti-mouse IgG (H&L) secondary antibody (HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for RPL18A Antibody (YA4170)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y (lane2, 40μg), HepG2 (lane3, 40μg), HEK293 (lane4, 20μg), MOLT-4 (lane5, 20μg) and Raji (lane6, 40μg) using RPL18A Antibody (HY-P84473). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 2 hour at room temperature.
  • Experimental Validation Results for RPL18A Antibody (YA4170)
  • Experimental Validation Results for RPL18A Antibody (YA4170)
  • RPL18A Antibody (YA4170) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 1), SH-SY5Y (lane 2), HeLa (lane 3), HepG2 (lane 4), A549 (lane 5), and Caco-2 (lane 6) using RPL18A antibody. Proteins were transferred onto a 0.45 μm NC membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody β-actin (HY-P80438) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane was incubated with HRP-conjugated goat anti-mouse IgG (H&L) secondary antibody (HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • RPL18A Antibody (YA4170) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SH-SY5Y (lane2, 40μg), HepG2 (lane3, 40μg), HEK293 (lane4, 20μg), MOLT-4 (lane5, 20μg) and Raji (lane6, 40μg) using RPL18A Antibody (HY-P84473). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 2 hour at room temperature.

  • RPL18A Antibody (YA4170) Product Image WB
  • RPL18A Antibody (YA4170) Product Image WB