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MEK3+MEK6 Antibody (YA3323)

(Synonyms: MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; MAP2K6; MEK3; MAP kinase kinase 3; MAPKK3; MAPK/ERK kinase 3)
Customer Review

Based on 1 Customer Validation

MEK3+MEK6 Antibody (YA3323) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MEK3+MEK6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB FC ICC
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
  • MEK3+MEK6 Antibody (YA3323) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 200-1:1000 1:100-1:200 1:20-1:100

Product Details

Description

MEK3+MEK6 Antibody (YA3323) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MEK3+MEK6.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 39,37 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39,37 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human MEK3/MEK6

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
    Western blot analysis of extracts from Hela(lane2(20μg), Jurkat(20μg), NIH/3T3(lane4(20μg) and K562(lane5(20μg) using MEK3+MEK6 Antibody (HY-P83578). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
    Flow cytometric analysis of 1X106 Jurkat cells labeling MEK3+MEK6 Antibody (HY-P83578, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
    Immunocytochemistry analysis of HeLa cells labeling MEK3+MEK6 with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
    Immunocytochemistry analysis of HepG2 cells labeling MEK3+MEK6 with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    MEK3+MEK6 is a P46734: Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14
    P52564: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6.

  • Subcellular Localization

    P52564: Nucleus; Cytoplasm; Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity: P46734: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues
    P52564: Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas
    Induction: P52564: Strongly activated by UV, anisomycin, and osmotic shock but not by phorbol esters, NGF or EGF

  • Subunit

    P46734: Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK (PubMed:21224381). Binds to DYRK1B/MIRK and increases its kinase activity (PubMed:11980910). Part of a complex with MAP3K3, RAC1 and CCM2 (By similarity). Interacts with ARRB1 (PubMed:16709866)
    P52564: Dimer. Interacts (via its D domain) with its substrates MAPK11, MAPK12, MAPK13 and MAPK14 (By similarity).

  • SwissProt ID

    P46734 / P52564

  • Gene ID
    5606 [NCBI] / 5608 [NCBI]
  • Synonyms

    MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; MAP2K6; MEK3; MAP kinase kinase 3; MAPKK3; MAPK/ERK kinase 3

  • Research Field

    Signal Transduction

MEK3+MEK6 Antibody (YA3323) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane2(20μg), Jurkat(20μg), NIH/3T3(lane4(20μg) and K562(lane5(20μg) using MEK3+MEK6 Antibody (HY-P83578). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling MEK3+MEK6 Antibody (HY-P83578, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling MEK3+MEK6 with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling MEK3+MEK6 with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MEK3+MEK6 Antibody (YA3323) Product Image
    1/16
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
  • MEK3+MEK6 Antibody (YA3323) Product Image
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane2(20μg), Jurkat(20μg), NIH/3T3(lane4(20μg) and K562(lane5(20μg) using MEK3+MEK6 Antibody (HY-P83578). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling MEK3+MEK6 Antibody (HY-P83578, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling MEK3+MEK6 with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling MEK3+MEK6 with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
  • Experimental Validation Results for MEK3+MEK6 Antibody (YA3323)
  • MEK3+MEK6 Antibody (YA3323) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane2(20μg), Jurkat(20μg), NIH/3T3(lane4(20μg) and K562(lane5(20μg) using MEK3+MEK6 Antibody (HY-P83578). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • MEK3+MEK6 Antibody (YA3323) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 Jurkat cells labeling MEK3+MEK6 Antibody (HY-P83578, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MEK3+MEK6 Antibody (YA3323) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling MEK3+MEK6 with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MEK3+MEK6 Antibody (YA3323) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling MEK3+MEK6 with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MEK3+MEK6 Antibody (HY-P83578) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MEK3+MEK6 Antibody (YA3323) Product Image WB
  • MEK3+MEK6 Antibody (YA3323) Product Image FC
  • MEK3+MEK6 Antibody (YA3323) Product Image ICC
  • MEK3+MEK6 Antibody (YA3323) Product Image ICC