S100 alpha Antibody (YA3316)

Cat. No.: HY-P83571: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

S100 alpha Antibody (YA3316) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to S100 alpha.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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S100 alpha Antibody (YA3316) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

S100 alpha Antibody (YA3316) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to S100 alpha.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 11 kDa;

Observed band size: 11 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P23297

Gene ID

6271 [NCBI]

Immunogen

A synthesized peptide derived from human S100 aa1-94.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:50
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from RAW264.7 (lane 2(20μg) and RAW264.7 (lane 3(40μg) using S100 alpha (HY-P83571) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed:23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446)

Subcellular Localization：Cytoplasm; Sarcoplasmic reticulum; Mitochondrion

Expression：
Tissue_specificity:It is abundant in the heart (PubMed: 12804600, PubMed: 1384693) . Small amounts have also been found in skeletal muscle and the brain (PubMed: 1384693) .

Subunit：Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain (PubMed:21296671). Also forms heterodimers with S100P (PubMed:15171681).

RRID

AB_3105679

Database

Entrez Gene: 6271 Human ; 20193 Mouse ; 295214 Rat

SwissProt: P23297 Human ; P56565 Mouse ; P35467 Rat

OMIM: 176940 Human

Research Field

Cell Biology

Synonyms

S100A1; S100A; Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

S100 alpha Antibody (YA3316) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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S100 alpha Antibody (YA3316)

S100 alpha Antibody (YA3316) Featured Recommendations:

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Background

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S100 alpha Antibody (YA3316) Related Classifications

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