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  5. S100 alpha Antibody (YA3316)

S100 alpha Antibody (YA3316)

Art. -Nr.: HY-P83571
COA
SDS
User Guide for Antibodies Technical Support

S100 alpha Antibody (YA3316) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to S100 alpha.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Beschreibung

Beschreibung

S100 alpha Antibody (YA3316) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to S100 alpha.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molekulargewicht
Predicted band size: 11 kDa;
Observed band size: 11 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human S100 aa1-94.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IP
IP: Immunoprecipitation
1:50
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Reinheit Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from RAW264.7 (lane 2(20μg) and RAW264.7 (lane 3(40μg) using S100 alpha (HY-P83571) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed:23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446)
Subcellular Localization：Cytoplasm; Sarcoplasmic reticulum; Mitochondrion
Expression：
Tissue_specificity:It is abundant in the heart (PubMed: 12804600, PubMed: 1384693) . Small amounts have also been found in skeletal muscle and the brain (PubMed: 1384693) .
Subunit：Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain (PubMed:21296671). Also forms heterodimers with S100P (PubMed:15171681).
RRID
Database

Entrez Gene: 6271 Human ; 20193 Mouse ; 295214 Rat

SwissProt: P23297 Human ; P56565 Mouse ; P35467 Rat

OMIM: 176940 Human

Research Field

Cell Biology
Synonyms
S100A1; S100A; Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1
Dokumentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

S100 alpha Antibody (YA3316) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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S100 alpha Antibody (YA3316)
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