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RF1 Antibody (YA4125)

(Synonyms: ERF; ETF1; ERF1; TB3-1; D5S1995; SUP45L1)
Customer Review

Based on 1 Customer Validation

RF1 Antibody (YA4125) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RF1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for RF1 Antibody (YA4125)
  • Experimental Validation Results for RF1 Antibody (YA4125)
  • Experimental Validation Results for RF1 Antibody (YA4125)
  • Experimental Validation Results for RF1 Antibody (YA4125)
  • RF1 Antibody (YA4125) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

RF1 Antibody (YA4125) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RF1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 49 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 49 kDa
Immunogen

Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718903

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RF1 Antibody (YA4125)
    Western blot analysis of extracts from MCF-7 (lane 2(20μg), Raji (lane 3(20μg), Hela (lane 4(20μg), HepG2 (lane 5(20μg) using RF1 Antibody（HY-P84428). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RF1 Antibody (YA4125)
    Western blot analysis of extracts from Rat liver(lane 1(40μg) )
  • Experimental Validation Results for RF1 Antibody (YA4125)
    Flow cytometric analysis of 1X10^6 Hela cells labeling RF1 Antibody(HY-P84428
  • Experimental Validation Results for RF1 Antibody (YA4125)
    Flow cytometric analysis of 1X10^6 HepG2 cells labeling RF1 Antibody(HY-P84428

Background

  • Function

    RF1 component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons. The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA. Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes[1][2][3][4][5][6][7][8][9].

  • Subcellular Localization

    Cytoplasm

  • Isoforms & Post-Translational Modification

    P62495 has 2 isomers: P62495-1: 49031 Da (predicted); P62495-2: 45463 Da (predicted).
    Methylated at Gln-185 by N6AMT1;Hydroxylation at Lys-63 by JMJD4 promotes its translational termination efficiency;Ubiquitinated at Lys-279 via 'Lys-6'-linked polyubiquitin chains by RNF14 and RNF25 in response to ribosome collisions (ribosome stalling), leading to its degradation by the proteasome and rescue of stalled ribosomes

  • Subunit

    Component of the eRF1-eRF3-GTP ternary complex, composed of ETF1/ERF1 and eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) and GTP (PubMed:19417105, PubMed:27863242). Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex (PubMed:19417104). Interacts with JMJD4 (PubMed:24486019). The ETF1-GSPT1 complex interacts with JMJD4 (PubMed:24486019)

  • SwissProt ID

    P62495

  • Gene ID
    2107 [NCBI]
  • Synonyms

    ERF; ETF1; ERF1; TB3-1; D5S1995; SUP45L1

RF1 Antibody (YA4125) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2(20μg), Raji (lane 3(20μg), Hela (lane 4(20μg), HepG2 (lane 5(20μg) using RF1 Antibody（HY-P84428). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Rat liver(lane 1(40μg) )

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling RF1 Antibody(HY-P84428

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HepG2 cells labeling RF1 Antibody(HY-P84428

  • RF1 Antibody (YA4125) Product Image
    1/16
  • Experimental Validation Results for RF1 Antibody (YA4125)
  • Experimental Validation Results for RF1 Antibody (YA4125)
  • Experimental Validation Results for RF1 Antibody (YA4125)
  • Experimental Validation Results for RF1 Antibody (YA4125)
  • RF1 Antibody (YA4125) Product Image
  • Experimental Validation Results for RF1 Antibody (YA4125)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2(20μg), Raji (lane 3(20μg), Hela (lane 4(20μg), HepG2 (lane 5(20μg) using RF1 Antibody（HY-P84428). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RF1 Antibody (YA4125)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Rat liver(lane 1(40μg) )
  • Experimental Validation Results for RF1 Antibody (YA4125)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling RF1 Antibody(HY-P84428
  • Experimental Validation Results for RF1 Antibody (YA4125)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HepG2 cells labeling RF1 Antibody(HY-P84428
  • Experimental Validation Results for RF1 Antibody (YA4125)
  • Experimental Validation Results for RF1 Antibody (YA4125)
  • Experimental Validation Results for RF1 Antibody (YA4125)
  • Experimental Validation Results for RF1 Antibody (YA4125)
  • RF1 Antibody (YA4125) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7 (lane 2(20μg), Raji (lane 3(20μg), Hela (lane 4(20μg), HepG2 (lane 5(20μg) using RF1 Antibody（HY-P84428). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • RF1 Antibody (YA4125) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Rat liver(lane 1(40μg) )

  • RF1 Antibody (YA4125) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling RF1 Antibody(HY-P84428

  • RF1 Antibody (YA4125) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 HepG2 cells labeling RF1 Antibody(HY-P84428

  • RF1 Antibody (YA4125) Product Image WB
  • RF1 Antibody (YA4125) Product Image WB
  • RF1 Antibody (YA4125) Product Image FC
  • RF1 Antibody (YA4125) Product Image FC