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beta Glucuronidase Antibody (YA2853)

(Synonyms: GUSB; Beta-glucuronidase; Beta-G1)
Customer Review

Based on 1 Customer Validation

beta Glucuronidase Antibody (YA2853) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to beta Glucuronidase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB FC ICC
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
  • beta Glucuronidase Antibody (YA2853) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

beta Glucuronidase Antibody (YA2853) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to beta Glucuronidase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 78 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 75 kDa
Species Reactivity Database

Entrez Gene: 2990 Human

SwissProt: P08236 Human

Immunogen

A synthesized peptide derived from human GUSB aa50-94.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105026

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
    Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using beta Glucuronidase Antibody (HY-P83108) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
    Flow cytometric analysis of 1X106 K562 cells labeling beta Glucuronidase Antibody (HY-P83108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
    Immunocytochemistry analysis of HepG2 cells labeling beta Glucuronidase Antibody (HY-P83108) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta Glucuronidase Antibody (HY-P83108) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
    Immunocytochemistry analysis of HepG2 cells labeling beta Glucuronidase Antibody (HY-P83108) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta Glucuronidase Antibody (HY-P83108) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    beta Glucuronidase plays an important role in the degradation of dermatan and keratan sulfates

  • Subcellular Localization

    Lysosome

  • Isoforms & Post-Translational Modification

    P08236 has 3 isomers: P08236-1: 74732 Da (predicted); P08236-2: 69142 Da (predicted); P08236-3: 58345 Da (predicted).
    N-linked glycosylated with 3 to 4 oligosaccharide chains

  • Subunit

    Homotetramer

  • SwissProt ID

    P08236

  • Gene ID
    2990 [NCBI]
  • Synonyms

    GUSB; Beta-glucuronidase; Beta-G1

  • Research Field

    -

beta Glucuronidase Antibody (YA2853) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using beta Glucuronidase Antibody (HY-P83108) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling beta Glucuronidase Antibody (HY-P83108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling beta Glucuronidase Antibody (HY-P83108) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta Glucuronidase Antibody (HY-P83108) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling beta Glucuronidase Antibody (HY-P83108) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta Glucuronidase Antibody (HY-P83108) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • beta Glucuronidase Antibody (YA2853) Product Image
    1/16
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
  • beta Glucuronidase Antibody (YA2853) Product Image
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using beta Glucuronidase Antibody (HY-P83108) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling beta Glucuronidase Antibody (HY-P83108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling beta Glucuronidase Antibody (HY-P83108) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta Glucuronidase Antibody (HY-P83108) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling beta Glucuronidase Antibody (HY-P83108) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta Glucuronidase Antibody (HY-P83108) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
  • Experimental Validation Results for beta Glucuronidase Antibody (YA2853)
  • beta Glucuronidase Antibody (YA2853) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20ug) and K562(lane 3(40ug) using beta Glucuronidase Antibody (HY-P83108) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • beta Glucuronidase Antibody (YA2853) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 K562 cells labeling beta Glucuronidase Antibody (HY-P83108, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • beta Glucuronidase Antibody (YA2853) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling beta Glucuronidase Antibody (HY-P83108) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta Glucuronidase Antibody (HY-P83108) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • beta Glucuronidase Antibody (YA2853) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling beta Glucuronidase Antibody (HY-P83108) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta Glucuronidase Antibody (HY-P83108) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • beta Glucuronidase Antibody (YA2853) Product Image WB
  • beta Glucuronidase Antibody (YA2853) Product Image FC
  • beta Glucuronidase Antibody (YA2853) Product Image ICC
  • beta Glucuronidase Antibody (YA2853) Product Image ICC