DGKA Antibody (YA2861)(PBS only)

Cat. No.: HY-P83116A: Data Sheet User Guide for Antibodies
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DGKA Antibody (YA2861) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DGKA.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DGKA Antibody (YA2861) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DGKA.

Host

Rabbit

Molecular Weight

Predicted band size: 83 kDa;

Observed band size: 83 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P23743

Gene ID

1606 [NCBI]

Immunogen

Recombinant protein of human DGKA aa511-700.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-1:2000
IP IP: Immunoprecipitation	1:20-1:50

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed:2175712). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:15544348, PubMed:2175712). Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group (PubMed:15544348). Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol (PubMed:22627129)

Subcellular Localization：Cytoplasm, cytosol

Expression：
Tissue_specificity:Expressed in lymphocytes

Isoforms & Post-Translational Modification：P23743 has 3 isomers: P23743-1: 82630 Da (predicted); P23743-2: 13486 Da (predicted); P23743-3: 28094 Da (predicted).

Subunit：Monomer

Research Field

Signal Transduction

Synonyms

DAGK; DAGK1; DGK-alpha

Documentation

User Guide for Antibodies (1077 KB)