Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody

製品番号: HY-P80814: データシート SDS COA User Guide for Antibodies
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Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187).

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容量	価格（税別）	在庫状況	数量
20 μL	$126	在庫あり	Estimated Time of Arrival: December 31
50 μL	$185	在庫あり	Estimated Time of Arrival: December 31
100 μL	$300	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

顧客検証

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187).

Host

Rabbit

Clonality

Polyclonal

分子量

Predicted band size: 44/42 kDa;

Observed band size: 42/44 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P27361 / P28482

Gene ID

5594 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of Human Phospho-p44/42 MAPK (Erk1/2).The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Sensitivity	Endogenous	純度	affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from NIH/3T3(lane 2(20ug) and NIH/3T3(lane 3(40ug) using Phospho-ERK1/2 Antibody (HY-P80814) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：P27361: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:34497368). MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.
P28482: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.

Subcellular Localization：P27361: Cytoplasm; Nucleus; Membrane, caveola; Cell junction, focal adhesion
P28482: Cytoplasm, cytoskeleton, spindle; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm; Membrane, caveola; Cell junction, focal adhesion

Subunit：P27361: Binds both upstream activators and downstream substrates in multimolecular complexes. Found in a complex with at least BRAF, HRAS, MAP2K1/MEK1, MAPK3 and RGS14 (By similarity).
P28482: Binds both upstream activators and downstream substrates in multimolecular complexes. This interaction inhibits its tyrosine-kinase activity.

RRID

AB_3102206

Database

Entrez Gene: 5594 Human ; 5595 Human ; 26413 Mouse ; 26417 Mouse ;

SwissProt: P27361 Human ; P28482 Human ; P63085 Mouse ; Q63844 Mouse ;

OMIM: 601795 Human

Research Field

Cell Biology

Synonyms

MAPK1/MAPK3

ドキュメンテーション

Select Batch:

データシート (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)