KARS Antibody (YA4958)
(Synonyms: KARS; KIAA0070; Lysine--tRNA ligase; Lysyl-tRNA synthetase; LysRS)KARS Antibody (YA4958) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to KARS.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB; IHC-P; IHC-F; ICC/IF; IF-Tissue; IP
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Reactivity :
Human, Mouse
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-200 | 1:100-200 | 1:50-200 | 1:50-200 | 1:20-50 |
Product Details
KARS Antibody (YA4958) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to KARS.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse Predicted Reactivity: Rat
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
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Observed Molecular WeightObserved band size: 73 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 68 kDa
A synthesized peptide derived from human Lysine tRNA ligase. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity; (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation[1][2][3][4][5][6][7][8].
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Subcellular Localization
Cytoplasm, cytosol; Cytoplasm; Nucleus; Cell membrane; Peripheral membrane protein; Secreted; Mitochondrion
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Isoforms & Post-Translational Modification
Q15046 has 2 isomers: Q15046-1: 68048 Da (predicted); Q15046-2: 71497 Da (predicted).
Phosphorylated on a serine residue after mast cell stimulation with immunoglobulin E (IgE) -
Subunit
Homodimer and tetradimer (PubMed:18272479, PubMed:23159739, PubMed:26074468, PubMed:28887846). Part of the multisynthetase complex (MSC), a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 (PubMed:19131329, PubMed:19289464, PubMed:23159739, PubMed:24312579). Interacts with AIMP2 (via N-terminus) and MITF (PubMed:14975237, PubMed:15220430, PubMed:23159739, PubMed:26074468, PubMed:31116475, PubMed:9878398). Interacts with TARSL2 (PubMed:24312579)
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SwissProt ID
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Synonyms
KARS; KIAA0070; Lysine--tRNA ligase; Lysyl-tRNA synthetase; LysRS
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