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IL-13 Receptor alpha 1 Antibody (YA4138)

(Synonyms: IL13RA1; NR4; CT19; IL-13Ra)
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Based on 1 Customer Validation

IL-13 Receptor alpha 1 Antibody (YA4138) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IL-13 Receptor alpha 1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for IL-13 Receptor alpha 1 Antibody (YA4138)
  • IL-13 Receptor alpha 1 Antibody (YA4138) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

IL-13 Receptor alpha 1 Antibody (YA4138) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IL-13 Receptor alpha 1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 49 kDa
Immunogen

Purified recombinant fragment of human CD213A1 (AA: extra 22-343) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IL-13 Receptor alpha 1 Antibody (YA4138)
    Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2), A549 (lane 3), Raji (lane 4), and HT-1080 (lane 5) using IL-13 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    IL-13 Receptor alpha 1 binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is widely distributed. The highest concentrations are found in the heart, liver, skeletal muscle, and ovaries; the lowest concentrations are found in the brain, lungs, and kidneys. It is also present in B cells, T cells, and endothelial cells.

  • Isoforms & Post-Translational Modification

    P78552 has 2 isomers: P78552-1: 48760 Da (predicted); P78552-2: 31659 Da (predicted).

  • Subunit

    Interleukin-13 receptor is a complex of IL4R, IL13RA1, and possibly other components. Interacts with TRAF3IP1. Interacts with IL4 (PubMed:18243101)

  • SwissProt ID

    P78552

  • Gene ID
    3597 [NCBI]
  • Synonyms

    IL13RA1; NR4; CT19; IL-13Ra

IL-13 Receptor alpha 1 Antibody (YA4138) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2), A549 (lane 3), Raji (lane 4), and HT-1080 (lane 5) using IL-13 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • IL-13 Receptor alpha 1 Antibody (YA4138) Product Image
    1/16
  • Experimental Validation Results for IL-13 Receptor alpha 1 Antibody (YA4138)
  • IL-13 Receptor alpha 1 Antibody (YA4138) Product Image
  • Experimental Validation Results for IL-13 Receptor alpha 1 Antibody (YA4138)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2), A549 (lane 3), Raji (lane 4), and HT-1080 (lane 5) using IL-13 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for IL-13 Receptor alpha 1 Antibody (YA4138)
  • IL-13 Receptor alpha 1 Antibody (YA4138) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2), A549 (lane 3), Raji (lane 4), and HT-1080 (lane 5) using IL-13 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • IL-13 Receptor alpha 1 Antibody (YA4138) Product Image WB