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Dnmt3b Antibody (YA1806)

(Synonyms: Dnmt3b; DNA methyltransferase HsaIIIB; DNMT3B)
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Based on 1 Customer Validation

Dnmt3b Antibody (YA1806) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Dnmt3b.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
  • Dnmt3b Antibody (YA1806) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Dnmt3b Antibody (YA1806) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Dnmt3b.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 97 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 96 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Dnmt3b aa55-111.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104181

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
    Western blot analysis of extracts from Hela(lane 2(20ug) , C6(lane 3(20ug) and HEK293(lane 4(20ug) using Dnmt3b Antibody (HY-P82061) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
    Immunocytochemistry analysis of Hela cells labeling Dnmt3b Antibody (HY-P82061) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Dnmt3b Antibody (HY-P82061) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
    Immunocytochemistry analysis of Hela cells labeling Dnmt3b Antibody (HY-P82061) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Dnmt3b Antibody (HY-P82061) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Dnmt3b is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells[1].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Universally present; highly expressed in fetal liver, heart, kidney, and placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isomer 1 is expressed in all tissues except brain, skeletal muscle, and peripheral blood mononuclear cells; isomer 3 is universally present; isomer 4 is expressed in all tissues except brain, skeletal muscle, lung, and prostate; isomer 5 is detected only in the testes, with extremely low expression levels in the brain and prostate.

  • Isoforms & Post-Translational Modification

    Q9UBC3 has 8 isomers: Q9UBC3-1: 95751 Da (predicted); Q9UBC3-2: 93407 Da (predicted); Q9UBC3-3: 86177 Da (predicted); Q9UBC3-4: 80845 Da (predicted); Q9UBC3-5: 88572 Da (predicted); Q9UBC3-6: 94689 Da (predicted); Q9UBC3-7: 77719 Da (predicted); Q9UBC3-8: 81311 Da (predicted).
    Sumoylated;Citrullinated by PADI4

  • Subunit

    Interacts with BAZ2A/TIP5, SUV39H1 and CBX4. Interacts with UHRF1 (By similarity). Interacts with DNMT1 and DNMT3A, SETDB1, UBL1, UBE2I9 and ZHX1. Interacts with the PRC2/EED-EZH2 complex

  • SwissProt ID

    Q9UBC3

  • Gene ID
    1789 [NCBI]
  • Synonyms

    Dnmt3b; DNA methyltransferase HsaIIIB; DNMT3B

  • Research Field

    Epigenetics and Nuclear Signaling

Dnmt3b Antibody (YA1806) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , C6(lane 3(20ug) and HEK293(lane 4(20ug) using Dnmt3b Antibody (HY-P82061) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Dnmt3b Antibody (HY-P82061) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Dnmt3b Antibody (HY-P82061) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Dnmt3b Antibody (HY-P82061) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Dnmt3b Antibody (HY-P82061) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Dnmt3b Antibody (YA1806) Product Image
    1/16
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
  • Dnmt3b Antibody (YA1806) Product Image
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , C6(lane 3(20ug) and HEK293(lane 4(20ug) using Dnmt3b Antibody (HY-P82061) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Dnmt3b Antibody (HY-P82061) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Dnmt3b Antibody (HY-P82061) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Dnmt3b Antibody (HY-P82061) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Dnmt3b Antibody (HY-P82061) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
  • Experimental Validation Results for Dnmt3b Antibody (YA1806)
  • Dnmt3b Antibody (YA1806) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , C6(lane 3(20ug) and HEK293(lane 4(20ug) using Dnmt3b Antibody (HY-P82061) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Dnmt3b Antibody (YA1806) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Dnmt3b Antibody (HY-P82061) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Dnmt3b Antibody (HY-P82061) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Dnmt3b Antibody (YA1806) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Dnmt3b Antibody (HY-P82061) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Dnmt3b Antibody (HY-P82061) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Dnmt3b Antibody (YA1806) Product Image WB
  • Dnmt3b Antibody (YA1806) Product Image ICC
  • Dnmt3b Antibody (YA1806) Product Image ICC