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GPRC5B Antibody (YA2964)(PBS only)

(Synonyms: RAIG2; RAIG-2)

GPRC5B Antibody (YA2964) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GPRC5B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GPRC5B Antibody (YA2964)(PBS only)
  • GPRC5B Antibody (YA2964)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:8000 1:20

Product Details

Description

GPRC5B Antibody (YA2964) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GPRC5B.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Immunogen

A synthetic peptide of human GPRC5B aa363-376.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GPRC5B Antibody (YA2964)(PBS only)
    Western blot analysis of extracts from 3T3 (lane 2(20μg)) , A549 (lane 3(20μg)), Jurkat(lane 4(20μg)) and Daudi (lane 5(20μg)) using GPRC5B Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    GPRC5B is a G-protein coupled receptor involved in the regulation of cell volume

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Cytoplasmic vesicle membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Expression is high in kidney, pancreas, and testis, medium in brain, heart, prostate, small intestine, and spleen, low in liver, placenta, skeletal muscle, colon, ovary, and thymus, and not detectable in lung and peripheral leukocyte. According to PubMed:10945465, highly expressed in most brain areas examined, with the highest levels observed in corpus callosum, caudate nucleus, putamen, substantia nigra, thalamus, hippocampus, and spinal cord as well as in dorsal root ganglia (DRG) . Expressed in glia limitans, ependymal cells, astrocyte cell bodies, the perivascular region in astrocyte endfeet, but not in neurons (PubMed:37143309) . In the periphery, expression levels are relatively low, compared to the CNS, with the strongest expression detected in pancreas, testis, uterus, and stomach

    Induction:By all-trans retinoic acid (ATRA)

  • Isoforms & Post-Translational Modification

    Q9NZH0 has 2 isomers: Q9NZH0-1: 44795 Da (predicted); Q9NZH0-2: 44933 Da (predicted).

  • SwissProt ID

    Q9NZH0

  • Gene ID
    51704 [NCBI]
  • Synonyms

    RAIG2; RAIG-2

  • Research Field

    Neuroscience

GPRC5B Antibody (YA2964)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from 3T3 (lane 2(20μg)) , A549 (lane 3(20μg)), Jurkat(lane 4(20μg)) and Daudi (lane 5(20μg)) using GPRC5B Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GPRC5B Antibody (YA2964)(PBS only) Product Image
    1/16
  • Experimental Validation Results for GPRC5B Antibody (YA2964)(PBS only)
  • GPRC5B Antibody (YA2964)(PBS only) Product Image
  • Experimental Validation Results for GPRC5B Antibody (YA2964)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from 3T3 (lane 2(20μg)) , A549 (lane 3(20μg)), Jurkat(lane 4(20μg)) and Daudi (lane 5(20μg)) using GPRC5B Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GPRC5B Antibody (YA2964)(PBS only)
  • GPRC5B Antibody (YA2964)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from 3T3 (lane 2(20μg)) , A549 (lane 3(20μg)), Jurkat(lane 4(20μg)) and Daudi (lane 5(20μg)) using GPRC5B Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GPRC5B Antibody (YA2964)(PBS only) Product Image WB