2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Adipose Triglyceride Lipase Antibody (YA637)

Adipose Triglyceride Lipase Antibody (YA637)

Cat. No.: HY-P80533
COA
SDS
User Guide for Antibodies Technical Support

Adipose Triglyceride Lipase Antibody (YA637) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Adipose Triglyceride Lipase.

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño Precio Stock Cantidad
20 μL En stock
50 μL En stock
100 μL En stock
250 μL   Obtener un presupuesto  
or Consulta para venta a granel

* Seleccione Cantidad antes de agregar artículos.

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Adipose Triglyceride Lipase Antibody (YA637)

  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Descripciòn

Descripciòn

Adipose Triglyceride Lipase Antibody (YA637) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Adipose Triglyceride Lipase.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Peso molecular
Predicted band size: 55 kDa;
Observed band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to Human Adipose Triglyceride Lipase.The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Pureza affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from HEK293 (lane 2(20μg), HepG2 (lane 3(20μg), A431 (lane 4(20μg) and SiHa (lane 5(20μg) using Adipose Triglyceride Lipase Antibody (HY-P80533). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15364929, PubMed:15550674, PubMed:16150821, PubMed:16239926, PubMed:17603008, PubMed:34903883). Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone and acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade (By similarity). Also possesses acylglycerol transacylase and phospholipase A2 activities (PubMed:15364929, PubMed:17032652, PubMed:17603008). Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides (PubMed:17603008). Regulates adiposome size and may be involved in the degradation of adiposomes (PubMed:16239926). Catalyzes the formation of an ester bond between hydroxy fatty acids and fatty acids derived from triglycerides or diglycerides to generate fatty acid esters of hydroxy fatty acids (FAHFAs) in adipocytes (PubMed:35676490). Acts antagonistically with LDAH in regulation of cellular lipid stores (PubMed:28578400). Inhibits LDAH-stimulated lipid droplet fusion (PubMed:28578400). May play an important role in energy homeostasis (By similarity). May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion (By similarity)
Subcellular Localization：Lipid droplet; Cell membrane; Multi-pass membrane protein; Cytoplasm
Expression：
Tissue_specificity:It is expressed most strongly in adipose tissue. It has also been detected in the heart, skeletal muscle, and parts of the gastrointestinal tract. It can be detected in both normal retina and retinoblastoma cells. It is also detected in the retinal pigment epithelium, with lower expression levels (protein levels) in photoreceptor ganglia and the neuroretinal ganglion cell layer.
Isoforms & Post-Translational Modification：Q96AD5 has 2 isomers: Q96AD5-1: 55316 Da (predicted); Q96AD5-2: 19875 Da (predicted).
Phosphorylation at Ser-404 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity (By similarity). Phosphorylation at Ser-404 is increased upon beta-adrenergic stimulation (PubMed:22733971);Ubiquitinated by PEX2 in response to reactive oxygen species (ROS), leading to its degradation (PubMed:34903883). Ubiquitination is stimulated by LDAH (PubMed:28578400)
Subunit：Interacts with ABHD5; this association stimulates PNPLA2 triglyceride hydrolase activity (By similarity). Interacts with SERPINF1; this interaction stimulates the phospholipase A2 activity of PNPLA2 (PubMed:17032652). Despite a colocalization in lipid droplets, it probably does not interact with PLIN (By similarity). Interacts with PLIN5; prevents interaction with ABHD5 (By similarity). Interacts with FAF2 (PubMed:23297223)
RRID
Database

Entrez Gene: 57104 Human ; 66853 Mouse ; 361676 Rat

SwissProt: Q96AD5 Human ; Q8BJ56 Mouse ; P0C548 Rat

OMIM: 610717 Human

Research Field

Cardiovascular
Synonyms
ATGL; Desnutrin; plpl; plpl2; Pnpla2; TTS 2.2; TTS2; TTS2.2; ZETA
Documentación
SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Adipose Triglyceride Lipase Antibody (YA637) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Productos vistos recientemente:

Consulta en línea

Your information is safe with us. * Required Fields.

Nombre del producto

  

Requested Quantity *

Nombre del solicitante *

 

Saludo

Direcciòn del E-mail *

 

Número de teléfono *

Department

 

Nombre de la Organizaciòn *

City

Provincia

Country or Region *

      

Observaciones

Consulta para venta a granel

Inquiry Information

Nombre del producto:
Adipose Triglyceride Lipase Antibody (YA637)
Cat. No.:
HY-P80533
Cantidad:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Nombre del producto

  

Lot #

Nombre del solicitante *

 

Country or Region *

Direcciòn del E-mail *

     

Número de teléfono *

 

Nombre de la Organizaciòn *

Observaciones

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Korean - KR (252 KB) Portuguese - PT (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.