Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)

Cat. No.: HY-P80464: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Hormone sensitive lipase (Ser853).

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Hormone sensitive lipase (Ser853).

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 117 kDa;

Observed band size: 90 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q05469

Gene ID

3991 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser853 of Human LIPE.AA range:821-870.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg)) and 3T3-L1(lane 4(20μg) or lane 5(40μg)), using Phospho-Hormone sensitive lipase (Ser853) (HY-P80464) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80464, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed:15716583, PubMed:15955102, PubMed:19800417, PubMed:8812477). Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs (PubMed:19800417). Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs (By similarity). Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (By similarity)

Subcellular Localization：Cell membrane; Membrane, caveola; Cytoplasm, cytosol; Lipid droplet

Expression：
Tissue_specificity:testis

Subunit：Monomer and homodimer (By similarity). Interacts with CAVIN1 in the adipocyte cytoplasm (PubMed:17026959). Interacts with PLIN5 (By similarity)

RRID

AB_3102538

Database

Entrez Gene: 3991 Human ; 16890 Mouse ; 25330 Rat

SwissProt: Q05469 Human ; P54310 Mouse ; P15304 Rat

OMIM: 615980 Human

Research Field

Cardiovascular

Synonyms

Hormone sensitive lipase antibody; Hormone sensitive lipase testicular isoform antibody; Hormone-sensitive lipase antibody; HSL antibody; LHS; Lipase hormone sensitive antibody; LIPE antibody; LIPS_HUMAN antibody; Phospho-Hormonesensitivelipase(S853); PhosphoHormonesensitivelipase(S853)

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)

Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) Featured Recommendations:

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