Copy Link Print Information

Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)

(Synonyms: Hormone sensitive lipase antibody; Hormone sensitive lipase testicular isoform antibody; Hormone-sensitive lipase antibody; HSL antibody; LHS; Lipase hormone sensitive antibody; LIPE antibody; LIPS_HUMAN antibody; Phospho-Hormonesensitivelipase(S853); PhosphoHormonesensitivelipase(S853) )
Customer Review

Based on 1 Customer Validation

Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Hormone sensitive lipase (Ser853).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)
  • Experimental Validation Results for Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)
  • Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:2000 1:50-1:200

Product Details

Description

Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Hormone sensitive lipase (Ser853).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 117 kDa
Species Reactivity Database
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser853 of Human LIPE.AA range:821-870.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102538

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)
    Western blot analysis was performed on protein extracts (25 μg) from NIH/3T3 (lane 2) and Mouse muscle (lane 3) using Phospho-Hormone sensitive lipase (Ser853) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% BSA in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% BSA in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)
    Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg)) and 3T3-L1(lane 4(20μg) or lane 5(40μg)), using Phospho-Hormone sensitive lipase (Ser853) (HY-P80464) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80464, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Hormone sensitive lipase is a Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production[1][2][3][4].

  • Subcellular Localization

    Cell membrane; Membrane, caveola; Cytoplasm, cytosol; Lipid droplet

  • Expression


    Tissue_specificity:testis

  • Subunit

    Monomer and homodimer (By similarity). Interacts with CAVIN1 in the adipocyte cytoplasm (PubMed:17026959). Interacts with PLIN5 (By similarity)

  • SwissProt ID

    Q05469

  • Gene ID
    3991 [NCBI]
  • Synonyms

    Hormone sensitive lipase antibody; Hormone sensitive lipase testicular isoform antibody; Hormone-sensitive lipase antibody; HSL antibody; LHS; Lipase hormone sensitive antibody; LIPE antibody; LIPS_HUMAN antibody; Phospho-Hormonesensitivelipase(S853); PhosphoHormonesensitivelipase(S853)

  • Research Field

    Cardiovascular

References

[1]. Ali YB, et al. Continuous monitoring of cholesterol oleate hydrolysis by hormone-sensitive lipase and other cholesterol esterases. J Lipid Res. 2005 May;46(5):994-1000. [Content Brief]

[2]. Gao J, et al. Identification of a novel keratinocyte retinyl ester hydrolase as a transacylase and lipase. J Invest Dermatol. 2005 Jun;124(6):1259-66. [Content Brief]

[3]. Rodriguez JA, et al. In vitro stereoselective hydrolysis of diacylglycerols by hormone-sensitive lipase. Biochim Biophys Acta. 2010 Jan;1801(1):77-83. [Content Brief]

[4]. Holst LS, et al. Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase. Genomics. 1996 Aug 1;35(3):441-7. [Content Brief]

View More

Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from NIH/3T3 (lane 2) and Mouse muscle (lane 3) using Phospho-Hormone sensitive lipase (Ser853) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% BSA in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% BSA in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg)) and 3T3-L1(lane 4(20μg) or lane 5(40μg)), using Phospho-Hormone sensitive lipase (Ser853) (HY-P80464) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80464, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) Product Image
    1/16
  • Experimental Validation Results for Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)
  • Experimental Validation Results for Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)
  • Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) Product Image
  • Experimental Validation Results for Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from NIH/3T3 (lane 2) and Mouse muscle (lane 3) using Phospho-Hormone sensitive lipase (Ser853) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% BSA in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% BSA in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg)) and 3T3-L1(lane 4(20μg) or lane 5(40μg)), using Phospho-Hormone sensitive lipase (Ser853) (HY-P80464) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80464, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)
  • Experimental Validation Results for Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189)
  • Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from NIH/3T3 (lane 2) and Mouse muscle (lane 3) using Phospho-Hormone sensitive lipase (Ser853) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% BSA in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% BSA in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg)) and 3T3-L1(lane 4(20μg) or lane 5(40μg)), using Phospho-Hormone sensitive lipase (Ser853) (HY-P80464) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80464, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) Product Image WB
  • Phospho-Hormone sensitive lipase (Ser853) Antibody (YA189) Product Image WB