SCAP Antibody (YA7221)

Cat. No.: HY-P87537: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

SCAP Antibody (YA7221) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to SCAP.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SCAP Antibody (YA7221) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to SCAP.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 140 kDa;

Observed band size: 120 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q12770

Immunogen

KLH conjugated synthetic peptide derived from human SCAP: 41-140/1279.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Sensitivity	Endogenous	Purity	affinity purified by Protein G
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1,k

Appearance

Liquid

Formulation

Supplied in 0.01M tris buffered saline (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Escort protein required for cholesterol as well as lipid homeostasis (By similarity). Regulates export of the SCAP-SREBP complex from the endoplasmic reticulum to the Golgi upon low cholesterol, thereby regulating the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed:26311497). At high sterol concentrations, formation of a ternary complex with INSIG (INSIG1 or INSIG2) leads to mask the ER export signal in SCAP, promoting retention of the complex in the endoplasmic reticulum (By similarity). Low sterol concentrations trigger release of INSIG, a conformational change in the SSD domain of SCAP, unmasking of the ER export signal, promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi: in the Golgi, SREBPs are then processed, releasing the transcription factor fragment of SREBPs from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (PubMed:26311497). Binds cholesterol via its SSD domain (By similarity)

Subcellular Localization：Endoplasmic reticulum membrane,Golgi apparatus membrane,Cytoplasmic vesicle, COPII-coated vesicle membrane

Expression：Induction: By androgen-bound AR and glucocorticoid-bound NR3C1 in a prostate cancer cell line (LNCaP)

Isoforms & Post-Translational Modification：Q12770 has four isomers: Q12770-1: 139729 Da (predicted); Q12770-2: 97903 Da (predicted); Q12770-3: 84773 Da (predicted); Q12770-4: 95616 Da (predicted).
Ubiquitinated at Lys-454 and Lys-466

Subunit：Membrane region forms a homotetramer (By similarity)

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)