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SCAP Antibody (YA7221)

(Synonyms: KIAA0199, PSEC0227, SCAP, Sterol regulatory element-binding protein cleavage-activating protein, SREBP cleavage-activating protein)
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SCAP Antibody (YA7221) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to SCAP.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 0.01M tris buffered saline (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • SCAP Antibody (YA7221) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-2000

Product Details

Description

SCAP Antibody (YA7221) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to SCAP.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 120 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 140 kDa
Immunogen

KLH conjugated synthetic peptide derived from human SCAP: 41-140/1279.

Sensitivity

Endogenous

Purification

affinity purified by Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M tris buffered saline (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    SCAP is an Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP-SREBP complex from the endoplasmic reticulum to the Golgi upon low cholesterol, thereby regulating the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2. At high sterol concentrations, formation of a ternary complex with INSIG (INSIG1 or INSIG2) leads to mask the ER export signal in SCAP, promoting retention of the complex in the endoplasmic reticulum. Low sterol concentrations trigger release of INSIG, a conformational change in the SSD domain of SCAP, unmasking of the ER export signal, promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi: in the Golgi, SREBPs are then processed, releasing the transcription factor fragment of SREBPs from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. Binds cholesterol via its SSD domain[1].

  • Subcellular Localization

    Endoplasmic reticulum membrane,Golgi apparatus membrane,Cytoplasmic vesicle, COPII-coated vesicle membrane

  • Expression

    Induction: By androgen-bound AR and glucocorticoid-bound NR3C1 in a prostate cancer cell line (LNCaP)

  • Isoforms & Post-Translational Modification

    Q12770 has four isomers: Q12770-1: 139729 Da (predicted); Q12770-2: 97903 Da (predicted); Q12770-3: 84773 Da (predicted); Q12770-4: 95616 Da (predicted).
    Ubiquitinated at Lys-454 and Lys-466

  • Subunit

    Membrane region forms a homotetramer (By similarity)

  • SwissProt ID

    Q12770

  • Synonyms

    KIAA0199, PSEC0227, SCAP, Sterol regulatory element-binding protein cleavage-activating protein, SREBP cleavage-activating protein

SCAP Antibody (YA7221) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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