Copy Link Print Information

Phospho-eIF2α (Ser51) Antibody (YA7608)

(Synonyms: EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, eIF2-alpha)
Customer Review

Based on 1 Customer Validation

Phospho-eIF2α (Ser51) Antibody (YA7608) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-eIF2α (Ser51).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat, Chicken, Pig, Dog

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Phospho-eIF2α (Ser51) Antibody (YA7608) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:100-1:1000 1:200-1:1000 1:5000-1:20000

Product Details

Description

Phospho-eIF2α (Ser51) Antibody (YA7608) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-eIF2α (Ser51).

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat, Chicken, Pig, Dog
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    eIF2α is a Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (43S PIC). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B. EIF2S1/eIF2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming. EIF2S1/eIF2-alpha also acts as an activator of mitophagy in response to mitochondrial damage: phosphorylation by EIF2AK1/HRI promotes relocalization to the mitochondrial surface, thereby triggering PRKN-independent mitophagy[1][2][3][4].

  • Subcellular Localization

    Cytoplasm, Stress granule,Cytoplasm, cytosol,Mitochondrion

  • Expression

    Induction: Up-regulated upon endoplasmic reticulum stress

  • Isoforms & Post-Translational Modification

    P05198: 315 amino acids, molecular weight 36112 Da.

  • Subunit

    Eukaryotic translation initiation factor 2 eIF2 is a heterotrimeric complex composed of an alpha (EIF2S1), a beta (EIF2S2) and a gamma (EIF2S3) chain (PubMed:23063529, PubMed:31048492, PubMed:31836389, PubMed:35031321)

  • SwissProt ID

    P05198

  • Synonyms

    EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, eIF2-alpha

References

[1]. Mikami S, et al. An efficient mammalian cell-free translation system supplemented with translation factors. Protein Expr Purif. 2006 Apr;46(2):348-57. [Content Brief]

[2]. Chakrabarty Y, et al. The HRI branch of the integrated stress response selectively triggers mitophagy. Mol Cell. 2024 Mar 21;84(6):1090-1100.e6. [Content Brief]

[3]. Lee YY, et al. An upstream open reading frame regulates translation of GADD34 during cellular stresses that induce eIF2alpha phosphorylation. J Biol Chem. 2009 Mar 13;284(11):6661-73. [Content Brief]

[4]. You K, et al. QRICH1 dictates the outcome of ER stress through transcriptional control of proteostasis. Science. 2021 Jan 1;371(6524):. [Content Brief]

View More

Phospho-eIF2α (Ser51) Antibody (YA7608) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • Phospho-eIF2α (Ser51) Antibody (YA7608) Product Image
    1/16
  • Phospho-eIF2α (Ser51) Antibody (YA7608) Product Image