Copy Link Print Information

Transferrin Receptor Antibody (YA840)

(Synonyms: Apotransferrin; beta 1 metal binding globulin; serotransferrin; TF)
Customer Review

Based on 1 Customer Validation

Transferrin Receptor Antibody (YA840) is a non-conjugated and Mouse origined IgG1 monoclonal antibody. It can be used as a loading control antibody.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Transferrin Receptor Antibody (YA840)
  • Transferrin Receptor Antibody (YA840) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
You May Still Need
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:5000-1:10000 1:50-1:200 1:100-1:200

Product Details

Description

Transferrin Receptor Antibody (YA840) is a non-conjugated and Mouse origined IgG1 monoclonal antibody. It can be used as a loading control antibody.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 77 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 77 kDa
Species Reactivity Database

Entrez Gene: 7037 Human

SwissProt: P02786 Human

Immunogen

Synthetic peptide corresponding to Transferrin.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103005

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Transferrin Receptor Antibody (YA840)
    Western blot analysis of extracts from HY-P70620 (lane 2(25 ng) and HY-P70620 (lane 3(50 ng) using Transferrin (HY-P80956) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Transferrin Receptor is a transmembrane glycoprotein. Transferrin Receptor consists of two isoforms, TfR1 and TfR2. Transferrin Receptor mediates the uptake of transferrin-iron complexes. Transferrin Receptor plays an important role in iron homeostasis as a gatekeeper regulating transferrin iron uptake. Transferrin Receptor is also considered as a proliferation marker and an attractive target for anticancer agents (e.g., a marker of poor prognosis in breast cancer) . Transferrin Receptor is associated with a variety of cancers including acute leukemia and breast cancer[1][2][3][4][5][6].

  • Subcellular Localization

    Cell membrane; Single-pass type II membrane protein; Melanosome; Secreted

  • Expression


    Induction:Regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation

  • Subunit

    Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with STEAP3

  • SwissProt ID

    P02786

  • Gene ID
    7037 [NCBI]
  • Synonyms

    Apotransferrin; beta 1 metal binding globulin; serotransferrin; TF

[1]. Marsee DK, et al. CD71 (transferrin receptor): an effective marker for erythroid precursors in bone marrow biopsy specimens. Am J Clin Pathol. 2010 Sep;134(3):429-35. [Content Brief]

[2]. Liu Q, et al. Significance of CD71 expression by flow cytometry in diagnosis of acute leukemia. Leuk Lymphoma. 2014 Apr;55(4):892-8. [Content Brief]

[3]. Dong HY, et al. CD71 is selectively and ubiquitously expressed at high levels in erythroid precursors of all maturation stages: a comparative immunochemical study with glycophorin A and hemoglobin A. Am J Surg Pathol. 2011 May;35(5):723-32. [Content Brief]

[4]. Habashy HO, et al. Transferrin receptor (CD71) is a marker of poor prognosis in breast cancer and can predict response to tamoxifen. Breast Cancer Res Treat. 2010 Jan;119(2):283-93. [Content Brief]

[5]. Hagag AA, et al. Prognostic Value of Transferrin Receptor-1 (CD71) Expression in Acute Lymphoblastic Leukemia. Endocr Metab Immune Disord Drug Targets. 2018;18(6):610-617. [Content Brief]

[6]. Kleven MD, et al. Transferrin Receptors TfR1 and TfR2 Bind Transferrin through Differing Mechanisms. Biochemistry. 2018 Mar 6;57(9):1552-1559. [Content Brief]

View More

Transferrin Receptor Antibody (YA840) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HY-P70620 (lane 2(25 ng) and HY-P70620 (lane 3(50 ng) using Transferrin (HY-P80956) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Transferrin Receptor Antibody (YA840) Product Image
    1/16
  • Experimental Validation Results for Transferrin Receptor Antibody (YA840)
  • Transferrin Receptor Antibody (YA840) Product Image
  • Experimental Validation Results for Transferrin Receptor Antibody (YA840)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HY-P70620 (lane 2(25 ng) and HY-P70620 (lane 3(50 ng) using Transferrin (HY-P80956) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Transferrin Receptor Antibody (YA840)
  • Transferrin Receptor Antibody (YA840) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HY-P70620 (lane 2(25 ng) and HY-P70620 (lane 3(50 ng) using Transferrin (HY-P80956) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Transferrin Receptor Antibody (YA840) Product Image WB