Transferrin Receptor Antibody (YA840)

Cat. No.: HY-P80956: Ficha de datos COA
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User Guide for Antibodies
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Transferrin Receptor Antibody (YA840) is a non-conjugated and Mouse origined IgG1 monoclonal antibody. It can be used as a loading control antibody.

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Tamaño	Stock	Cantidad
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10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
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Transferrin Receptor Antibody (YA840) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

Transferrin Receptor Antibody (YA840) is a non-conjugated and Mouse origined IgG1 monoclonal antibody. It can be used as a loading control antibody.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 77 kDa;

Observed band size: 77 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P02786

Gene ID

7037 [NCBI]

Immunogen

Synthetic peptide corresponding to Transferrin.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000-1:10000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:200

Sensitivity	Endogenous	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HY-P70620 (lane 2(25 ng) and HY-P70620 (lane 3(50 ng) using Transferrin (HY-P80956) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738). Mediates uptake of NICOL1 into fibroblasts where it may regulate extracellular matrix production (By similarity); (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus; (Microbial infection) Acts as a host entry factor for rabies virus that hijacks the endocytosis of TFRC to enter cells; (Microbial infection) Acts as a host entry factor for SARS-CoV, MERS-CoV and SARS-CoV-2 viruses that hijack the endocytosis of TFRC to enter cells

Subcellular Localization：Cell membrane; Single-pass type II membrane protein; Melanosome; Secreted

Expression：
Induction:Regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation

Subunit：Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with STEAP3

RRID

AB_3103005

Database

Entrez Gene: 7037 Human

SwissProt: P02786 Human

OMIM: 616740 Human

Synonyms

Apotransferrin; beta 1 metal binding globulin; serotransferrin; TF

Documentación

Select Batch:

Ficha de datos (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Transferrin Receptor Antibody (YA840) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Transferrin Receptor Antibody (YA840)

Transferrin Receptor Antibody (YA840) Recomendaciones destacadas:

Product Detail

Verification Image

Background

Descripciòn

Transferrin Receptor Antibody (YA840) Related Classifications

Productos vistos recientemente:

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