VAP-1 Antibody (YA8965)
(Synonyms: VAP1, AOC3, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1, SSAO, VAP-1)VAP-1 Antibody (YA8965) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to VAP-1.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IF-Tissue, FC, IP
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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FC
FC: Flow Cytometry
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:100-1000 | 1:50-500 | 1:50-500 | 1μg per 1*106 cells | 1-2μg per 100-500μg Total protein |
Product Details
VAP-1 Antibody (YA8965) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to VAP-1.
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Host Mouse
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Species Reactivityhuman
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Observed Molecular WeightObserved band size: 110 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 84 kDa
OMIM: 603735
A synthesized peptide derived from human VAP-1.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
VAP-1 catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia. Has a preference for the primary monoamines methylamine and benzylamine. Could also act on 2-phenylethylamine but much less efficiently. At endothelial cells surface can also function as a cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion[1][2][3][4].
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Subcellular Localization
Cell membrane
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Expression
Tissue_Specificity: Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis. Isoform 2 seems to be the predominant transcript in fetal kidneys, fetal cartilage and fetal tonsils. The highest relative expression of isoform 2 occurs in skeletal muscle, heart, pancreas, kidney, and lung.
Induction: Up-regulated during in vitro adipocyte differentiation. -
Isoforms & Post-Translational Modification
VAP-1 has 3 isoforms, Q16853-1: amino acid length is 763, molecular weight is 84622 Da (predicted); Q16853-2: amino acid length is 634, molecular weight is 70675 Da (predicted); Q16853-3: amino acid length is 220, molecular weight is 24533 Da (predicted).
Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue -
Subunit
Homodimer; disulfide-linked.
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SwissProt ID
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Synonyms
VAP1, AOC3, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1, SSAO, VAP-1
Documentation
References
[1]. Kaitaniemi S, et al. The unique substrate specificity of human AOC2, a semicarbazide-sensitive amine oxidase. Cell Mol Life Sci. 2009 Aug;66(16):2743-57. [Content Brief]
[2]. Bligt-Lindén E, et al. Novel pyridazinone inhibitors for vascular adhesion protein-1 (VAP-1): old target-new inhibition mode. J Med Chem. 2013 Dec 27;56(24):9837-48. [Content Brief]
[3]. Smith DJ, et al. Cloning of vascular adhesion protein 1 reveals a novel multifunctional adhesion molecule. J Exp Med. 1998 Jul 6;188(1):17-27. [Content Brief]
[4]. Salmi M, et al. Vascular adhesion protein 1 (VAP-1) mediates lymphocyte subtype-specific, selectin-independent recognition of vascular endothelium in human lymph nodes. J Exp Med. 1997 Aug 18;186(4):589-600. [Content Brief]
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