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SRF Antibody (YA6778)

(Synonyms: c fos serum response element binding factor antibody; c fos serum response element binding transcription factor antibody; ELK3 antibody; ERP antibody; MCM 1 antibody; MCM1 antibody; OTTHUMP00000039820 antibody; SAP2 antibody; Serum response factor antibody; SRF antibody; c fos serum response element binding factor antibody; c fos serum response element binding transcription factor antibody; ELK3 antibody; ERP antibody; MCM 1 antibody; MCM1 antibody; OTTHUMP00000039820 antibody; SAP2 antibody; Serum response factor antibody; SRF antibody; SRF serum response factor c fos serum response element binding transcription factor antibody; SRF_HUMAN antibody; )
Customer Review

Based on 1 Customer Validation

SRF Antibody (YA6778) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SRF.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SRF Antibody (YA6778)
  • SRF Antibody (YA6778) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000 1:100 1:200-1:1000 1:1000 1-2μg/sample

Product Details

Description

SRF Antibody (YA6778) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SRF.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 67 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Immunogen

Recombinant protein within human SRF aa 1-400 / 508.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SRF Antibody (YA6778)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using SRF Antibody (HY-P87085) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    SRF is a SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    P11831: 508 amino acids, molecular weight 51593 Da.

  • Subunit

    Binds DNA as a multimer, probably a dimer (PubMed:7637780)

  • SwissProt ID

    P11831

  • Gene ID
    6722 [NCBI]
  • Synonyms

    c fos serum response element binding factor antibody; c fos serum response element binding transcription factor antibody; ELK3 antibody; ERP antibody; MCM 1 antibody; MCM1 antibody; OTTHUMP00000039820 antibody; SAP2 antibody; Serum response factor antibody; SRF antibody; c fos serum response element binding factor antibody; c fos serum response element binding transcription factor antibody; ELK3 antibody; ERP antibody; MCM 1 antibody; MCM1 antibody; OTTHUMP00000039820 antibody; SAP2 antibody; Serum response factor antibody; SRF antibody; SRF serum response factor c fos serum response element binding transcription factor antibody; SRF_HUMAN antibody;

SRF Antibody (YA6778) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using SRF Antibody (HY-P87085) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SRF Antibody (YA6778) Product Image
    1/16
  • Experimental Validation Results for SRF Antibody (YA6778)
  • SRF Antibody (YA6778) Product Image
  • Experimental Validation Results for SRF Antibody (YA6778)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using SRF Antibody (HY-P87085) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SRF Antibody (YA6778)
  • SRF Antibody (YA6778) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using SRF Antibody (HY-P87085) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SRF Antibody (YA6778) Product Image WB