Wnt-10b Antibody (YA5102)

Cat. No.: HY-P85410: Fiche technique COA
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Wnt-10b Antibody (YA5102) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Wnt-10b.

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Size	Stock	Quantité
Échantillon gratuit	Apply now
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Description

Description

Wnt-10b Antibody (YA5102) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Wnt-10b.

Host

Mouse

Clonality

Monoclonal,Recombinant

Masse moléculaire

Predicted band size: 45 kDa;

Species Reactivity

Human, Rat Predicted Reactivity: Mouse

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

O00744

Gene ID

7480 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human WNT10B. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-500

Sensitivity	Endogenous	Pureté	affinity purified by Protein G
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1, Kappa

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from MCF-7 (lane2(20μg), SH-SY5Y (lane3(20μg), Hela (lane4(20μg) and HCT116 (lane5(20μg) using Wnt-10b Antibody (HY-P85410). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

Background

Function：Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin

Subcellular Localization：Secreted, extracellular space, extracellular matrix; Secreted

Expression：
Tissue_specificity:It has been detected in most adult tissues. The highest concentrations are found in the heart and skeletal muscle. Lower concentrations are found in brain tissue.

Isoforms & Post-Translational Modification：O00744 has 2 isomers: O00744-1: 43000 Da (predicted); O00744-2: 20483 Da (predicted).
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition

Subunit：Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720). The complex with AFM may represent the physiological form in body fluids (PubMed:26902720)

Synonyms

WNT10B; WNT12; Protein Wnt-10b; Protein Wnt-12

Documentation

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Fiche technique (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)