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IL-4R Antibody(YA6869)

(Synonyms: CD124 antibody; IL 4R alpha antibody; IL-4 receptor subunit alpha antibody; IL-4-binding protein antibody; IL-4R subunit alpha antibody; IL-4R-alpha antibody; IL-4RA antibody; IL4-BP antibody; Il4r antibody; IL4R nirs variant 1 antibody; IL4RA antibody; IL4RA_HUMAN antibody; Interleukin 4 receptor alpha chain antibody; Interleukin 4 receptor antibody; sIL4Ralpha/prot antibody; Soluble IL-4 receptor subunit alpha antibody; Soluble IL-4R-alpha antibody)
Customer Review

Based on 1 Customer Validation

IL-4R Antibody(YA6869) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-4R.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for IL-4R Antibody(YA6869)
  • Experimental Validation Results for IL-4R Antibody(YA6869)
  • Experimental Validation Results for IL-4R Antibody(YA6869)
  • IL-4R Antibody(YA6869) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000 1:100 1-2μg/sample

Product Details

Description

IL-4R Antibody(YA6869) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-4R.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 90 kDa
Species Reactivity Database
Immunogen

Recombinant protein within human IL-4R aa 1-232.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719281

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IL-4R Antibody(YA6869)
    Western blot analysis of extracts from THP-1 (lane 2(20μg), HEK293T (lane 3(20μg), K562 (lane 4(20μg), MCF-7 (lane 5(20μg), using IL-4R Antibody（HY-P87177). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IL-4R Antibody(YA6869)
    Immunocytochemistry analysis of A431 cells labeling IL-4R with IL-4R Antibody (HY-P87177) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IL-4R Antibody (HY-P87177) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IL-4R Antibody(YA6869)
    Immunocytochemistry analysis of Hela cells labeling IL-4R with IL-4R Antibody (HY-P87177) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IL-4R Antibody (HY-P87177) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    IL-4R receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2|Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells[1].

  • Subcellular Localization

    Cell membrane,Secreted

  • Expression


    Tissue_Specificity: Isoform 1 and isoform 2 are highly expressed in activated T-cells

  • Isoforms & Post-Translational Modification

    P24394 has three isomers: P24394-1: 89658 Da (predicted); P24394-2: 25553 Da (predicted); P24394-3: 88267 Da (predicted).
    On IL4 binding, phosphorylated on C-terminal tyrosine residues丨The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase

  • Subunit

    The functional IL4 receptor is formed by initial binding of IL4 to IL4R

  • SwissProt ID

    P24394

  • Gene ID
    3566 [NCBI]
  • Synonyms

    CD124 antibody; IL 4R alpha antibody; IL-4 receptor subunit alpha antibody; IL-4-binding protein antibody; IL-4R subunit alpha antibody; IL-4R-alpha antibody; IL-4RA antibody; IL4-BP antibody; Il4r antibody; IL4R nirs variant 1 antibody; IL4RA antibody; IL4RA_HUMAN antibody; Interleukin 4 receptor alpha chain antibody; Interleukin 4 receptor antibody; sIL4Ralpha/prot antibody; Soluble IL-4 receptor subunit alpha antibody; Soluble IL-4R-alpha antibody

IL-4R Antibody(YA6869) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 2(20μg), HEK293T (lane 3(20μg), K562 (lane 4(20μg), MCF-7 (lane 5(20μg), using IL-4R Antibody（HY-P87177). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling IL-4R with IL-4R Antibody (HY-P87177) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IL-4R Antibody (HY-P87177) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling IL-4R with IL-4R Antibody (HY-P87177) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IL-4R Antibody (HY-P87177) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IL-4R Antibody(YA6869) Product Image
    1/16
  • Experimental Validation Results for IL-4R Antibody(YA6869)
  • Experimental Validation Results for IL-4R Antibody(YA6869)
  • Experimental Validation Results for IL-4R Antibody(YA6869)
  • IL-4R Antibody(YA6869) Product Image
  • Experimental Validation Results for IL-4R Antibody(YA6869)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 2(20μg), HEK293T (lane 3(20μg), K562 (lane 4(20μg), MCF-7 (lane 5(20μg), using IL-4R Antibody（HY-P87177). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IL-4R Antibody(YA6869)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling IL-4R with IL-4R Antibody (HY-P87177) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IL-4R Antibody (HY-P87177) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IL-4R Antibody(YA6869)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling IL-4R with IL-4R Antibody (HY-P87177) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IL-4R Antibody (HY-P87177) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IL-4R Antibody(YA6869)
  • Experimental Validation Results for IL-4R Antibody(YA6869)
  • Experimental Validation Results for IL-4R Antibody(YA6869)
  • IL-4R Antibody(YA6869) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from THP-1 (lane 2(20μg), HEK293T (lane 3(20μg), K562 (lane 4(20μg), MCF-7 (lane 5(20μg), using IL-4R Antibody（HY-P87177). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • IL-4R Antibody(YA6869) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling IL-4R with IL-4R Antibody (HY-P87177) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IL-4R Antibody (HY-P87177) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IL-4R Antibody(YA6869) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling IL-4R with IL-4R Antibody (HY-P87177) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IL-4R Antibody (HY-P87177) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IL-4R Antibody(YA6869) Product Image WB
  • IL-4R Antibody(YA6869) Product Image ICC
  • IL-4R Antibody(YA6869) Product Image ICC