IL-4R Antibody(YA6869)

Cat. No.: HY-P87177: Data Sheet COA
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IL-4R Antibody(YA6869) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-4R.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

IL-4R Antibody(YA6869) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-4R.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 90 kDa;

Observed band size: 110 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P24394

Gene ID

3566 [NCBI]

Immunogen

Recombinant protein within human IL-4R aa 1-232.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IP IP: Immunoprecipitation	1-2μg/sample

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from THP-1 (lane 2(20μg), HEK293T (lane 3(20μg), K562 (lane 4(20μg), MCF-7 (lane 5(20μg), using IL-4R Antibody（HY-P87177). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

Background

Function：Receptor for both interleukin 4 and interleukin 13 (PubMed:17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2|Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells

Subcellular Localization：Cell membrane,Secreted

Expression：
Tissue_Specificity: Isoform 1 and isoform 2 are highly expressed in activated T-cells

Isoforms & Post-Translational Modification：P24394 has three isomers: P24394-1: 89658 Da (predicted); P24394-2: 25553 Da (predicted); P24394-3: 88267 Da (predicted).
On IL4 binding, phosphorylated on C-terminal tyrosine residues丨The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase

Subunit：The functional IL4 receptor is formed by initial binding of IL4 to IL4R

RRID

AB_3719281

Database

SwissProt: P24394 Human ; P16382 Mouse ; Q63257 Rat

Synonyms

CD124 antibody; IL 4R alpha antibody; IL-4 receptor subunit alpha antibody; IL-4-binding protein antibody; IL-4R subunit alpha antibody; IL-4R-alpha antibody; IL-4RA antibody; IL4-BP antibody; Il4r antibody; IL4R nirs variant 1 antibody; IL4RA antibody; IL4RA_HUMAN antibody; Interleukin 4 receptor alpha chain antibody; Interleukin 4 receptor antibody; sIL4Ralpha/prot antibody; Soluble IL-4 receptor subunit alpha antibody; Soluble IL-4R-alpha antibody

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)