Toll-like receptor 5 Antibody (YA10018)
(Synonyms: TIL3, TLR5, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3)Toll-like receptor 5 Antibody (YA10018) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Toll-like receptor 5.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, ICC/IF
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
Toll-like receptor 5 Antibody (YA10018) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Toll-like receptor 5.
-
Host Rabbit
-
Clonality Monoclonal,Recombinant
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 97 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 98 kDa
Synthetic peptide corresponding to human TLR5 protein.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Toll-like receptor 5 is a Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life (By similarity)[1][2][3][4][5].
-
Subcellular Localization
Cell membrane
-
Expression
Tissue_Specificity: Highly expressed on the basolateral surface of intestinal epithelia. Expressed also in other cells such as lung epithelial cells. -
Isoforms & Post-Translational Modification
Toll-like receptor 5 has an amino acid length of 858, molecular weight is 97800 Da.
Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines. -
Subunit
Homodimer.
-
SwissProt ID
-
Synonyms
TIL3, TLR5, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3
Documentation
References
[1]. Hayashi F, et al. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 2001 Apr 26;410(6832):1099-103. [Content Brief]
[2]. Blohmke CJ, et al. Innate immunity mediated by TLR5 as a novel antiinflammatory target for cystic fibrosis lung disease. J Immunol. 2008 Jun 1;180(11):7764-73. [Content Brief]
[3]. Bielaszewska M, et al. Enterohemorrhagic Escherichia coli O157 outer membrane vesicles induce interleukin 8 production in human intestinal epithelial cells by signaling via Toll-like receptors TLR4 and TLR5 and activation of the nuclear factor NF-κB. Int J Med Microbiol. 2018 Oct;308(7):882-889. [Content Brief]
[4]. Gewirtz AT, et al. Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression. J Immunol. 2001 Aug 15;167(4):1882-5. [Content Brief]
[5]. Choi YJ, et al. TRIF mediates Toll-like receptor 5-induced signaling in intestinal epithelial cells. J Biol Chem. 2010 Nov 26;285(48):37570-8. [Content Brief]
Powered by Bioz
