Transferrin Receptor Antibody (YA3546)
(Synonyms: CD71 antibody; IMD46 antibody; OTTHUMP00000208523 antibody; p90 antibody; sTfR antibody; T9 antibody; TFR 1 antibody; TfR antibody; TFRC antibody; TR antibody; Transferrin receptor (p90 CD71) antibody )
Based on 1 Customer Validation
Transferrin Receptor Antibody (YA3546) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transferrin Receptor.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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FC
FC: Flow Cytometry
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| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50 | 1:50-1:100 |
Product Details
Transferrin Receptor Antibody (YA3546) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transferrin Receptor.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 90 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 85 kDa
Entrez Gene: 7037 Human ; 22042 Mouse ; 64678 Rat
SwissProt: P02786 Human ; Q62351 Mouse ; Q99376 Rat
OMIM: 616740 Human
A synthesized peptide derived from human TFR.
Endogenous
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from K562(lane2(20μg), Jurkat (lane3(20μg), Hela (lane4(20μg) and HepG2 (lane5(20μg) using Transferrin Receptor Antibody (HY-P81000). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Transferrin Receptor 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Transferrin Receptor 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of 1X106 Jurkat cells labeling Transferrin Receptor Antibody (HY-P81000, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Background
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Function
Transferrin Receptor is a transmembrane glycoprotein. Transferrin Receptor consists of two isoforms, TfR1 and TfR2. Transferrin Receptor mediates the uptake of transferrin-iron complexes. Transferrin Receptor plays an important role in iron homeostasis as a gatekeeper regulating transferrin iron uptake. Transferrin Receptor is also considered as a proliferation marker and an attractive target for anticancer agents (e.g., a marker of poor prognosis in breast cancer) . Transferrin Receptor is associated with a variety of cancers including acute leukemia and breast cancer[1][2][3][4][5][6].
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Subcellular Localization
Cell membrane; Single-pass type II membrane protein; Melanosome; Secreted
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Expression
Induction:Regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation -
Subunit
Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with STEAP3
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SwissProt ID
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Synonyms
CD71 antibody; IMD46 antibody; OTTHUMP00000208523 antibody; p90 antibody; sTfR antibody; T9 antibody; TFR 1 antibody; TfR antibody; TFRC antibody; TR antibody; Transferrin receptor (p90 CD71) antibody
Documentation
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Data Sheet (261 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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User Guide for Antibodies (1077 KB)
[1]. Marsee DK, et al. CD71 (transferrin receptor): an effective marker for erythroid precursors in bone marrow biopsy specimens. Am J Clin Pathol. 2010 Sep;134(3):429-35. [Content Brief]
[2]. Liu Q, et al. Significance of CD71 expression by flow cytometry in diagnosis of acute leukemia. Leuk Lymphoma. 2014 Apr;55(4):892-8. [Content Brief]
[3]. Dong HY, et al. CD71 is selectively and ubiquitously expressed at high levels in erythroid precursors of all maturation stages: a comparative immunochemical study with glycophorin A and hemoglobin A. Am J Surg Pathol. 2011 May;35(5):723-32. [Content Brief]
[4]. Habashy HO, et al. Transferrin receptor (CD71) is a marker of poor prognosis in breast cancer and can predict response to tamoxifen. Breast Cancer Res Treat. 2010 Jan;119(2):283-93. [Content Brief]
[5]. Hagag AA, et al. Prognostic Value of Transferrin Receptor-1 (CD71) Expression in Acute Lymphoblastic Leukemia. Endocr Metab Immune Disord Drug Targets. 2018;18(6):610-617. [Content Brief]
[6]. Kleven MD, et al. Transferrin Receptors TfR1 and TfR2 Bind Transferrin through Differing Mechanisms. Biochemistry. 2018 Mar 6;57(9):1552-1559. [Content Brief]
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