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Heme Oxygenase 1 Antibody (YA6087)

(Synonyms: Heme oxygenase 1 (HO-1) (EC 1.14.99.3))
Customer Review

Based on 1 Customer Validation

Heme Oxygenase 1 Antibody (YA6087) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Heme Oxygenase 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Heme Oxygenase 1 Antibody (YA6087) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000-1:10000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Heme Oxygenase 1 Antibody (YA6087) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Heme Oxygenase 1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719145

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    Western blot analysis of extracts from A549 (lane 2(20μg), HEK293 (lane 3(20μg), RAW264.7 (lane 4(20μg), NIH/3T3 (lane 5(20μg) using Heme Oxygenase 1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    Immunohistochemical analysis of paraffin-embedded mouse duodenum tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Heme Oxygenase 1 catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis; (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy; Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron[1][2][3][4].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Single-pass type IV membrane protein; Cytoplasmic side

  • Expression


    Tissue_specificity:The expression level in renal cell carcinoma tissue is higher than that in normal tissue (protein level) .

    Induction:Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA

  • Subunit

    (Microbial infection) Interacts with SARS-CoV-2 ORF3A protein; the interaction promotes ORF3A-induced autophagy but is unlikely to be involved in ORF3A-mediated induction of reticulophagy

  • SwissProt ID

    P09601

  • Gene ID
    3162 [NCBI]
  • Synonyms

    Heme oxygenase 1 (HO-1) (EC 1.14.99.3)

References

[1]. Lightning LK, et al. Disruption of an active site hydrogen bond converts human heme oxygenase-1 into a peroxidase. J Biol Chem. 2001 Apr 6;276(14):10612-9. [Content Brief]

[2]. Hwang HW, et al. Oligomerization is crucial for the stability and function of heme oxygenase-1 in the endoplasmic reticulum. J Biol Chem. 2009 Aug 21;284(34):22672-9. [Content Brief]

[3]. Wilks A, et al. Expression and characterization of truncated human heme oxygenase (hHO-1) and a fusion protein of hHO-1 with human cytochrome P450 reductase. Biochemistry. 1995 Apr 4;34(13):4421-7. [Content Brief]

[4]. Gozzelino R, et al. Mechanisms of cell protection by heme oxygenase-1. Annu Rev Pharmacol Toxicol. 2010;50:323-54. [Content Brief]

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Heme Oxygenase 1 Antibody (YA6087) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg), HEK293 (lane 3(20μg), RAW264.7 (lane 4(20μg), NIH/3T3 (lane 5(20μg) using Heme Oxygenase 1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse duodenum tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Heme Oxygenase 1 Antibody (YA6087) Product Image
    1/16
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Heme Oxygenase 1 Antibody (YA6087) Product Image
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg), HEK293 (lane 3(20μg), RAW264.7 (lane 4(20μg), NIH/3T3 (lane 5(20μg) using Heme Oxygenase 1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse duodenum tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA6087)
  • Heme Oxygenase 1 Antibody (YA6087) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane 2(20μg), HEK293 (lane 3(20μg), RAW264.7 (lane 4(20μg), NIH/3T3 (lane 5(20μg) using Heme Oxygenase 1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Heme Oxygenase 1 Antibody (YA6087) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Heme Oxygenase 1 Antibody (YA6087) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Heme Oxygenase 1 Antibody (YA6087) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Heme Oxygenase 1 Antibody (YA6087) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Heme Oxygenase 1 Antibody (YA6087) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse duodenum tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Heme Oxygenase 1 Antibody (YA6087) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Heme Oxygenase 1 Antibody (HY-P86395, 1/8000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Heme Oxygenase 1 Antibody (YA6087) Product Image WB
  • Heme Oxygenase 1 Antibody (YA6087) Product Image IHC-P
  • Heme Oxygenase 1 Antibody (YA6087) Product Image IHC-P
  • Heme Oxygenase 1 Antibody (YA6087) Product Image IHC-P
  • Heme Oxygenase 1 Antibody (YA6087) Product Image IHC-P
  • Heme Oxygenase 1 Antibody (YA6087) Product Image IHC-P
  • Heme Oxygenase 1 Antibody (YA6087) Product Image IHC-P