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NQO1 Antibody (YA261)

(Synonyms: DIA4, NMOR1, NQO1, NAD(P)H dehydrogenase [quinone] 1, Azoreductase, DT-diaphorase, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, DTD, QR1)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

NQO1 Antibody (YA261) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NQO1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL ICC FC
  • Experimental Validation Results for NQO1 Antibody (YA261)
  • Experimental Validation Results for NQO1 Antibody (YA261)
  • Experimental Validation Results for NQO1 Antibody (YA261)
  • NQO1 Antibody (YA261) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:50-1:200 1:50-1:100 Use at an assay dependent concentration.

Product Details

Description

NQO1 Antibody (YA261) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NQO1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 31 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human NQO1.AA range:1-49.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102381

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NQO1 Antibody (YA261)
    Immunocytochemistry analysis of HepG2 cells labeling NQO1 with NQO1 Antibody (HY-P80251) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NQO1 Antibody (HY-P0251) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NQO1 Antibody (YA261)
    Immunocytochemistry analysis of Hela cells labeling NQO1 with NQO1 Antibody (HY-P80251) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NQO1 Antibody (HY-P80251)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NQO1 Antibody (YA261)
    Flow cytometric analysis of 1X10^6 MCF-7 cells labeling NQO1 Antibody (YA261) (HY-P80251, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    NQO1 (NAD (P) H:quinone oxidoreductase 1) is a cytosolic flavoprotein that catalyzes the obligate two-electron reduction of endogenous and exogenous quinones to hydroquinones, thereby limiting semiquinone formation, redox cycling, and reactive oxygen species generation[1][2]. Through this catalytic activity, NQO1 functions as a major cellular defense enzyme that supports redox homeostasis and protects cells from oxidative and electrophilic stress[1][3]. Mechanistically, NQO1 is a prominent downstream target of the Keap1-Nrf2-ARE signaling pathway, and its transcription is strongly induced during oxidative stress and xenobiotic exposure, linking environmental stress responses to cytoprotective gene expression programs[3][4]. In disease contexts, NQO1 is frequently overexpressed in multiple solid tumors, including lung, breast, liver, colon, and pancreatic cancers, where elevated expression is associated with adaptation to oxidative stress and altered responses to therapeutic agents[3][5][6]. Compared with the related isoform NQO2, NQO1 is the predominant stress-inducible quinone reductase and utilizes NADH or NADPH as reducing cofactors, whereas NQO2 exhibits distinct cofactor preferences and biological regulation, supporting nonredundant cellular functions[7][6]. For experimental applications, NQO1 is widely used as a model enzyme for investigating redox regulation, quinone metabolism, and cytoprotective signaling pathways, while the inhibitor dicoumarol remains a commonly employed pharmacological tool for probing NQO1-dependent biological mechanisms[2][6].

  • Subcellular Localization

    Cytoplasm, cytosol

  • Expression


    Induction:By dioxin (PubMed:1657151) . By oxidative stress (PubMed:21636573)

  • Subunit

    Homodimer (PubMed:10543876, PubMed:10706635, PubMed:11587640, PubMed:11735396, PubMed:16700548, PubMed:28291250). Interacts with PDLIM4 isoform 2; this interaction stabilizes PDLIM4 isoform 2 in response to oxidative stress and protects it from ubiquitin-independent degradation by the core 20S proteasome (PubMed:21636573). Interacts with TP73 (via SAM domain); this interaction is NADH-dependent, stabilizes TP73 in response to oxidative stress and protects it from ubiquitin-independent degradation by the 20S proteasome (PubMed:15687255, PubMed:28291250). Interacts with TP53; this interaction is NADH-dependent, stabilizes TP53 in response to oxidative stress and protects it from ubiquitin-independent degradation by the 20S proteasome (PubMed:15687255)

  • SwissProt ID

    P15559

  • Gene ID
    1728 [NCBI]
  • Synonyms

    DIA4, NMOR1, NQO1, NAD(P)H dehydrogenase [quinone] 1, Azoreductase, DT-diaphorase, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, DTD, QR1

  • Research Field

    Signal Transduction

[1]. Dinkova-Kostova AT, et al. NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1), a multifunctional antioxidant enzyme and exceptionally versatile cytoprotector. Arch Biochem Biophys. 2010 Sep 1;501(1):116-23. [Content Brief]

[2]. Bianchet MA, et al. Structure and mechanism of NAD[P]H:quinone acceptor oxidoreductases (NQO). Methods Enzymol. 2004;382:144-74. [Content Brief]

[3]. Lee WS, et al. Roles of NAD(P)H:quinone Oxidoreductase 1 in Diverse Diseases. Life (Basel). 2021 Nov 26;11(12):1301. [Content Brief]

[4]. Ross D, et al. Functions of NQO1 in Cellular Protection and CoQ10 Metabolism and its Potential Role as a Redox Sensitive Molecular Switch. Front Physiol. 2017 Aug 24;8:595. [Content Brief]

[5]. Yang Y, et al. Clinical implications of high NQO1 expression in breast cancers. J Exp Clin Cancer Res. 2014 Feb 5;33(1):14. [Content Brief]

[6]. Pey AL, et al. NAD(P)H quinone oxidoreductase (NQO1): an enzyme which needs just enough mobility, in just the right places. Biosci Rep. 2019 Jan 3;39(1):BSR20180459. [Content Brief]

[7]. Jaiswal AK, et al. Localization of human NQO1 gene to chromosome 16q22 and NQO2-6p25 and associated polymorphisms. Pharmacogenetics. 1999 Jun;9(3):413-8. [Content Brief]

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NQO1 Antibody (YA261) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling NQO1 with NQO1 Antibody (HY-P80251) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NQO1 Antibody (HY-P0251) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling NQO1 with NQO1 Antibody (HY-P80251) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NQO1 Antibody (HY-P80251)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 MCF-7 cells labeling NQO1 Antibody (YA261) (HY-P80251, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • NQO1 Antibody (YA261) Product Image
    1/16
  • Experimental Validation Results for NQO1 Antibody (YA261)
  • Experimental Validation Results for NQO1 Antibody (YA261)
  • Experimental Validation Results for NQO1 Antibody (YA261)
  • NQO1 Antibody (YA261) Product Image
  • Experimental Validation Results for NQO1 Antibody (YA261)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling NQO1 with NQO1 Antibody (HY-P80251) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NQO1 Antibody (HY-P0251) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NQO1 Antibody (YA261)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling NQO1 with NQO1 Antibody (HY-P80251) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NQO1 Antibody (HY-P80251)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NQO1 Antibody (YA261)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 MCF-7 cells labeling NQO1 Antibody (YA261) (HY-P80251, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for NQO1 Antibody (YA261)
  • Experimental Validation Results for NQO1 Antibody (YA261)
  • Experimental Validation Results for NQO1 Antibody (YA261)
  • NQO1 Antibody (YA261) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling NQO1 with NQO1 Antibody (HY-P80251) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NQO1 Antibody (HY-P0251) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NQO1 Antibody (YA261) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling NQO1 with NQO1 Antibody (HY-P80251) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NQO1 Antibody (HY-P80251)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NQO1 Antibody (YA261) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 MCF-7 cells labeling NQO1 Antibody (YA261) (HY-P80251, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • NQO1 Antibody (YA261) Product Image ICC
  • NQO1 Antibody (YA261) Product Image ICC
  • NQO1 Antibody (YA261) Product Image FC