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Neurotensin(8-13) 

Cat. No.: HY-P0251 Purity: >98.0%
Handling Instructions

Neurotensin (8-13) is an active fragment of Neurotensin,. Neurotensin(8-13) results in a decrease in cell-surface NT1 receptors (NTR1) density.

For research use only. We do not sell to patients.

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Neurotensin(8-13) Chemical Structure

Neurotensin(8-13) Chemical Structure

CAS No. : 60482-95-3

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Based on 1 publication(s) in Google Scholar

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Description

Neurotensin (8-13) is an active fragment of Neurotensin,. Neurotensin(8-13) results in a decrease in cell-surface NT1 receptors (NTR1) density.

IC50 & Target

NTR1[1]

In Vitro

Receptor internalization induced by Neurotensin(8-13) results in a decrease in cell-surface NT1 receptors (NTR1) density. The receptor downregulation in response to high extracellular concentrations of the peptide has been described for Neurotensin (NT) in HT-29 cells and in rat primary cultured neurons. Reappearance of the receptors on the cell surface is also different[1].

Molecular Weight

816.99

Formula

C₃₈H₆₄N₁₂O₈

CAS No.

60482-95-3

Sequence

Arg-Arg-Pro-Tyr-Ile-Leu

Sequence Shortening

RRPYIL

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (61.20 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2240 mL 6.1200 mL 12.2401 mL
5 mM 0.2448 mL 1.2240 mL 2.4480 mL
10 mM 0.1224 mL 0.6120 mL 1.2240 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[1]

Binding assays are performed on whole HT-29 cells at confluence. A day before the assay, cells (106 cells/0.4 mL, equivalent to 0.3 mg protein) are placed in 48-well plates. A special binding buffer that includes protease inhibitors (50 mM HEPES, 125 mM NaCl, 7.5 mM KCl, 5.5 mM MgCl2, 1 mM EGTA, 5 g/L bovine serum albumin, 2 mg/L chymostatin, 100 mg/L soybean trypsin inhibitor, 50 mg/L bacitracin, pH 7.4) is used for the experiments. In inhibition studies, cells are incubated for 1 h at 37°C in triplicate with 25,000 cpm of 125I-NT and variable concentrations (0.001-3,000 nM) of unlabeled NT(8-13), unlabeled NT-VIII, or NT-VIII labeled with natRe (final volume of 0.2 mL per well). The cells are then washed twice with cold binding buffer and afterward are solubilized with 1N NaOH at 37°C (0.4 mL per well). The activity is determined in a γ-counter. In saturation studies, cells are incubated in triplicate with increasing concentrations (0.1-10 nM) of 99mTc(CO)3NT-VIII for 1 h at 37°C (final volume, 0.2 mL per well). The concentrations of total technetium (99+99mTc) are equivalent to 0.2-20 MBq 99mTc activity per well. After 2 washings with the same binding buffer as before, the cells are then solubilized with 1N NaOH at 37°C (0.4 mL per well). The bound radioactivity is measured in the γ-counter. Nonspecific binding is determined with 1 μM unlabeled NT(8-13)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

Neurotensin(8-13)OthersInhibitorinhibitorinhibit

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Neurotensin(8-13)
Cat. No.:
HY-P0251
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