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TXNIP Antibody (YA024)

(Synonyms: VDUP1, TXNIP, Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

TXNIP Antibody (YA024) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TXNIP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC ICC
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • TXNIP Antibody (YA024) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:5000 1:50-1:200 1:50-1:400 1:50-1:100

Product Details

Description

TXNIP Antibody (YA024) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TXNIP.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 44 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human TXNIP.AA range:50-99.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3094610

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TXNIP Antibody (YA024)
    Western blot analysis of extracts from Hela (lane 2(20μg) or lane 3(40μg)) and NIH3T3 (lane 4(20μg) or lane 5(40μg)) using TXNIP (HY-P80361) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TXNIP Antibody (YA024)
    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using TXNIP Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80361, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TXNIP Antibody (YA024)
    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using TXNIP Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80361, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TXNIP Antibody (YA024)
    Flow cytometric analysis of 1X10^6 Hela cells labeling TXNIP Antibody (HY-P80361, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for TXNIP Antibody (YA024)
    Immunocytochemistry analysis of Hela cells labeling TXNIP with TXNIP Antibody (HY-P80361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TXNIP Antibody (HY-P80361) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TXNIP Antibody (YA024)
    Immunocytochemistry analysis of NIH-3T3 cells labeling TXNIP with TXNIP Antibody (HY-P80361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TXNIP Antibody (HY-P80361) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability (PubMed:17603038). Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest (PubMed:12821938). Required for the maturation of natural killer cells (By similarity). Acts as a suppressor of tumor cell growth (PubMed:18541147). Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (PubMed:21460850)

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Induction:By 1,25-dihydroxyvitamin D-3 and TGFB1. Down-regulated in response to oxidative stress

  • Subunit

    Homodimer; disulfide-linked (PubMed:23519408). Interacts with TXN/thioredoxin through its redox-active site (PubMed:17603038). Interacts with transcriptional repressors ZBTB16, ZBTB32 and HDAC1 (PubMed:12821938). Interacts with DDIT4 (PubMed:21460850)

  • SwissProt ID

    Q9H3M7

  • Gene ID
    10628 [NCBI]
  • Synonyms

    VDUP1, TXNIP, Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1

  • Research Field

    Signal Transduction

TXNIP Antibody (YA024) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) or lane 3(40μg)) and NIH3T3 (lane 4(20μg) or lane 5(40μg)) using TXNIP (HY-P80361) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using TXNIP Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80361, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using TXNIP Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80361, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling TXNIP Antibody (HY-P80361, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TXNIP with TXNIP Antibody (HY-P80361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TXNIP Antibody (HY-P80361) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling TXNIP with TXNIP Antibody (HY-P80361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TXNIP Antibody (HY-P80361) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TXNIP Antibody (YA024) Product Image
    1/16
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • TXNIP Antibody (YA024) Product Image
  • Experimental Validation Results for TXNIP Antibody (YA024)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) or lane 3(40μg)) and NIH3T3 (lane 4(20μg) or lane 5(40μg)) using TXNIP (HY-P80361) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TXNIP Antibody (YA024)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using TXNIP Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80361, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TXNIP Antibody (YA024)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using TXNIP Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80361, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TXNIP Antibody (YA024)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling TXNIP Antibody (HY-P80361, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for TXNIP Antibody (YA024)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TXNIP with TXNIP Antibody (HY-P80361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TXNIP Antibody (HY-P80361) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TXNIP Antibody (YA024)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling TXNIP with TXNIP Antibody (HY-P80361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TXNIP Antibody (HY-P80361) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • Experimental Validation Results for TXNIP Antibody (YA024)
  • TXNIP Antibody (YA024) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) or lane 3(40μg)) and NIH3T3 (lane 4(20μg) or lane 5(40μg)) using TXNIP (HY-P80361) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • TXNIP Antibody (YA024) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using TXNIP Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80361, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TXNIP Antibody (YA024) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using TXNIP Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80361, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TXNIP Antibody (YA024) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling TXNIP Antibody (HY-P80361, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • TXNIP Antibody (YA024) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling TXNIP with TXNIP Antibody (HY-P80361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TXNIP Antibody (HY-P80361) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TXNIP Antibody (YA024) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH-3T3 cells labeling TXNIP with TXNIP Antibody (HY-P80361) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TXNIP Antibody (HY-P80361) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TXNIP Antibody (YA024) Product Image WB
  • TXNIP Antibody (YA024) Product Image IHC-P
  • TXNIP Antibody (YA024) Product Image IHC-P
  • TXNIP Antibody (YA024) Product Image FC
  • TXNIP Antibody (YA024) Product Image ICC
  • TXNIP Antibody (YA024) Product Image ICC