NUMB Antibody (YA7750)

Cat. No.: HY-P88066: Data Sheet User Guide for Antibodies
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NUMB Antibody (YA7750) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NUMB.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NUMB Antibody (YA7750) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NUMB.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 69.3 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P49757

Immunogen

Full length human recombinant protein of human NUMB produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Regulates clathrin-mediated receptor endocytosis (PubMed:18657069). Plays a role in the process of neurogenesis (By similarity). Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate (By similarity). Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity (By similarity). May also mediate local repair of brain ventricular wall damage (By similarity)

Subcellular Localization：Cell membrane,Endosome membrane

Isoforms & Post-Translational Modification：P49757 has 9 isomers: P49757-1: 70804 Da (predicted); P49757-2: 65899 Da (predicted); P49757-3: 69431 Da (predicted); P49757-4: 64527 Da (predicted); P49757-5: 55319 Da (predicted); P49757-6: 53947 Da (predicted); P49757-7: 50198 Da (predicted); P49757-8: 48825 Da (predicted); P49757-9: 37396 Da (predicted).
Phosphorylated on Ser-276 and Ser-295 by CaMK1丨Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity)

Subunit：Interacts with SIAH1 (PubMed:11752454)

Synonyms

C14orf41; c14_5527; S171

Documentation

User Guide for Antibodies (1077 KB)