SAFB1 Antibody (YA1042)
(Synonyms: SAFB; HAP; HET; SAFB1; Scaffold attachment factor B1; SAF-B; SAF-B1; HSP27 estrogen response element-TATA box-binding protein; HSP27 ERE-TATA-binding protein)SAFB1 Antibody (YA1042) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SAFB1.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, ChIP
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Reactivity :
Human
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ChIP
ChIP: Chromatin Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 | 1:20 |
Product Details
SAFB1 Antibody (YA1042) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SAFB1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 130 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 103 kDa
Purified recombinant human SAFB1 protein fragments expressed in E.coli.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SAFB1 binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Thereby acts as a negative regulator of cell proliferation. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter[1][2].
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:It is widely distributed. It is highly expressed in the central nervous system and lowly expressed in the liver. It is also expressed in various breast cancer cell lines. -
Isoforms & Post-Translational Modification
Q15424 has 4 isomers: Q15424-1: 102642 Da (predicted); Q15424-2: 95181 Da (predicted); Q15424-3: 102855 Da (predicted); Q15424-4: 102768 Da (predicted).
Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity -
Subunit
Monomer and homodimer (PubMed:12660241). Forms heterodimers with SAFB2 (PubMed:12660241). Interacts with KHDRBS3 (By similarity). Interacts with CLK2 (By similarity). Interacts with POLR2A, SRSF1/ASF, SRSF9/SRp30c and SFSF10/TRA2B (PubMed:9671816). Interacts with isoform 1 and isoform 2 of SRPK1 and inhibits its activity (PubMed:19674106). Interacts with RBMX (By similarity). Interacts with FUS (PubMed:27731383). Interacts with ZBED4 (PubMed:22693546)
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SwissProt ID
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Synonyms
SAFB; HAP; HET; SAFB1; Scaffold attachment factor B1; SAF-B; SAF-B1; HSP27 estrogen response element-TATA box-binding protein; HSP27 ERE-TATA-binding protein
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Research Field
Epigenetics and Nuclear Signaling
Documentation
References
[1]. Nayler O, et al. SAF-B protein couples transcription and pre-mRNA splicing to SAR/MAR elements. Nucleic Acids Res. 1998 Aug 1;26(15):3542-9. [Content Brief]
[2]. Townson SM, et al. SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor. J Biol Chem. 2003 May 30;278(22):20059-68. [Content Brief]
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