Phospho-CRK (Tyr221) Antibody (YA10014)
(Synonyms: Adapter molecule crk, Proto-oncogene c-Crk, p38, CRK)Phospho-CRK (Tyr221) Antibody (YA10014) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Phospho-CRK (Tyr221).
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, ICC/IF
-
Reactivity :
Human
-
Formulation:
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
Phospho-CRK (Tyr221) Antibody (YA10014) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Phospho-CRK (Tyr221).
-
Host Rabbit
-
Clonality Monoclonal,Recombinant
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 34 kDa
Synthetic Phospho-peptide corresponding to residues surrounding Y221 of human CRK.
Endogenous
affinity purified.
Non-conjugated
Phosphorylated
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
CRK is involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1; Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling[1][2].
-
Subcellular Localization
Cytoplasm; Cell membrane
-
Isoforms & Post-Translational Modification
P46108 has 2 isomers: P46108-1: 33831 Da (predicted); P46108-2: 22906 Da (predicted).
Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway (PubMed:17515907). Isoform Crk-II: Phosphorylated by KIT (By similarity);Proline isomerization at Pro-237 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form -
Subunit
Component of a complex comprised of SH2D3C, BCAR1/CAS, and CRK (PubMed:12432078). Within the complex, interacts with SH2D3C (via C-terminus), and BCAR1/CAS (PubMed:12432078). Found in a complex with ABL1, ABL2, CRK and UNC119; leading to the inhibition of CRK phosphorylation by ABL kinases (PubMed:19381274). Interacts with ABL1, C3G, DOCK3, DOCK5, MAP4K1, MAPK8 and SOS via its first SH3 domain. Interacts (via SH2 domain) with BCAR1, CBL, CBLB, PXN, IRS4 and GAB1 upon stimulus-induced tyrosine phosphorylation. Interacts (via SH2 domain) with several tyrosine-phosphorylated growth factor receptors such as EGFR and INSR. Interacts with FLT1 (tyrosine-phosphorylated). Interacts with DOCK1 and DOCK4. Interacts with SHB. Interacts with PEAK1. Interacts with FASLG. Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and p130cas/BCAR1. Interacts (via SH2 domain) with the 'Tyr-9' phosphorylated form of PDPK1. Interacts with CBLC
-
SwissProt ID
-
Synonyms
Adapter molecule crk, Proto-oncogene c-Crk, p38, CRK
Documentation
Powered by Bioz
