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Myosin heavy chain Antibody (YA3200)

(Synonyms: MYH6; MYHC; MYHCA; MYHCB; Myosin heavy chain 6; Myosin heavy chain; Myosin-6; Myosin heavy chain; cardiac muscle alpha isoform)
Customer Review

Based on 1 Customer Validation

Myosin heavy chain Antibody (YA3200) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin heavy chain.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Myosin heavy chain Antibody (YA3200)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA3200)
  • Myosin heavy chain Antibody (YA3200) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Myosin heavy chain Antibody (YA3200) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin heavy chain.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 224 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 224 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Myosin heavy chain aa1100-1400.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105566

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Myosin heavy chain Antibody (YA3200)
    Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYH6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA3200)
    Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYH6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Myosin heavy chain is a Muscle contraction

  • Subcellular Localization

    Cytoplasm, myofibril

  • Subunit

    Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2)

  • SwissProt ID

    P13533

  • Gene ID
    4624 [NCBI]
  • Synonyms

    MYH6; MYHC; MYHCA; MYHCB; Myosin heavy chain 6; Myosin heavy chain; Myosin-6; Myosin heavy chain; cardiac muscle alpha isoform

  • Research Field

    Signal Transduction

Myosin heavy chain Antibody (YA3200) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYH6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYH6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myosin heavy chain Antibody (YA3200) Product Image
    1/16
  • Experimental Validation Results for Myosin heavy chain Antibody (YA3200)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA3200)
  • Myosin heavy chain Antibody (YA3200) Product Image
  • Experimental Validation Results for Myosin heavy chain Antibody (YA3200)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYH6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA3200)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYH6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myosin heavy chain Antibody (YA3200)
  • Experimental Validation Results for Myosin heavy chain Antibody (YA3200)
  • Myosin heavy chain Antibody (YA3200) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYH6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myosin heavy chain Antibody (YA3200) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYH6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myosin heavy chain Antibody (YA3200) Product Image IHC-P
  • Myosin heavy chain Antibody (YA3200) Product Image IHC-P