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Klotho Antibody (YA3355)

(Synonyms: Kl; Klotho peptide)
Customer Review

Based on 1 Customer Validation

Klotho Antibody (YA3355) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Klotho.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Klotho Antibody (YA3355)
  • Klotho Antibody (YA3355) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Klotho Antibody (YA3355) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Klotho.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 62,116 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62,116 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Klotho aa421-435.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105717

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Klotho Antibody (YA3355)
    Western blot analysis was performed on extracts from HL-60 (lane 1, 20 μg) using Klotho Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Klotho may have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1; The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Apical cell membrane; Single-pass type I membrane protein; Secreted; Secreted

  • Expression


    Tissue_specificity:It is present in the renal cortical tubules (protein level) . Soluble peptides are present in serum and cerebrospinal fluid. It is expressed in the kidney, placenta, small intestine, and prostate. Its expression is downregulated in the kidneys of renal cell carcinoma, hepatocellular carcinoma, and chronic renal failure.

  • Isoforms & Post-Translational Modification

    Q9UEF7 has 2 isomers: Q9UEF7-1: 116181 Da (predicted); Q9UEF7-2: 62135 Da (predicted).
    N-glycosylated

  • Subunit

    Homodimer. Interacts with FGF23 and FGFR1

  • SwissProt ID

    Q9UEF7

  • Gene ID
    9365 [NCBI]
  • Synonyms

    Kl; Klotho peptide

  • Research Field

    Signal Transduction

Klotho Antibody (YA3355) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HL-60 (lane 1, 20 μg) using Klotho Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Klotho Antibody (YA3355) Product Image
    1/16
  • Experimental Validation Results for Klotho Antibody (YA3355)
  • Klotho Antibody (YA3355) Product Image
  • Experimental Validation Results for Klotho Antibody (YA3355)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HL-60 (lane 1, 20 μg) using Klotho Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Klotho Antibody (YA3355)
  • Klotho Antibody (YA3355) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HL-60 (lane 1, 20 μg) using Klotho Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Klotho Antibody (YA3355) Product Image WB