Klotho Antibody (YA3355)

Cat. No.: HY-P83610: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Klotho Antibody (YA3355) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Klotho.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

Klotho Antibody (YA3355) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Klotho Antibody (YA3355) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Klotho.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 62,116 kDa;

Observed band size: 62,116 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q9UEF7

Gene ID

9365 [NCBI]

Immunogen

A synthesized peptide derived from human Klotho aa421-435.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from HL-60 (lane 1, 20 μg) using Klotho Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity); The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Apical cell membrane; Single-pass type I membrane protein; Secreted; Secreted

Expression：
Tissue_specificity:It is present in the renal cortical tubules (protein level) . Soluble peptides are present in serum and cerebrospinal fluid. It is expressed in the kidney, placenta, small intestine, and prostate. Its expression is downregulated in the kidneys of renal cell carcinoma, hepatocellular carcinoma, and chronic renal failure.

Isoforms & Post-Translational Modification：Q9UEF7 has 2 isomers: Q9UEF7-1: 116181 Da (predicted); Q9UEF7-2: 62135 Da (predicted).
N-glycosylated

Subunit：Homodimer. Interacts with FGF23 and FGFR1

RRID

AB_3105717

Database

Entrez Gene: 9365 Human ; 16591 Mouse ;

SwissProt: Q9UEF7 Human ; O35082 Mouse ;

OMIM: 617994 Human

Research Field

Signal Transduction

Synonyms

Kl; Klotho peptide

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Klotho Antibody (YA3355) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Country or Region *

Email Address *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Klotho Antibody (YA3355)

Klotho Antibody (YA3355) Featured Recommendations:

Product Detail

Verification Image

Background

Documentation

Klotho Antibody (YA3355) Related Classifications

Your Recently Viewed Products:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report