SIN1 Antibody (YA6980)

Cat. No.: HY-P87297: Data Sheet COA
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SIN1 Antibody (YA6980) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SIN1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

SIN1 Antibody (YA6980) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SIN1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 59 kDa;

Observed band size: 65/75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9BPZ7

Immunogen

Recombinant protein within human SIN1 aa 350-522.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Sensitivity	Endogenous	Purity	Protein G affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from MCF7 (lane 1(20μg)) 、A549 (lane 2(20μg)) 、HepG2 (lane 3(20μg)) and HeLa (lane 4(20μg)) using SIN1 Antibody (HY-P87297) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Component of the mechanistic target of rapamycin complex 2 (mTORC2), which transduces signals from growth factors to pathways involved in proliferation, cytoskeletal organization, lipogenesis and anabolic output (PubMed:15467718, PubMed:16919458, PubMed:16962653, PubMed:17043309, PubMed:21806543, PubMed:28264193, PubMed:28968999, PubMed:30837283, PubMed:35926713). In response to growth factors, mTORC2 phosphorylates and activates AGC protein kinase family members, including AKT (AKT1, AKT2 and AKT3), PKC (PRKCA, PRKCB and PRKCE) and SGK1 (PubMed:16919458, PubMed:16962653, PubMed:21806543, PubMed:28264193, PubMed:28968999, PubMed:30837283, PubMed:35926713). In contrast to mTORC1, mTORC2 is nutrient-insensitive (PubMed:16962653). Within the mTORC2 complex, MAPKAP1/SIN1 acts as a substrate adapter which recognizes and binds AGC protein kinase family members for phosphorylation by MTOR (PubMed:21806543, PubMed:28264193). mTORC2 plays a critical role in AKT1 activation by mediating phosphorylation of different sites depending on the context, such as 'Thr-450', 'Ser-473', 'Ser-477' or 'Thr-479', facilitating the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDPK1/PDK1 which is a prerequisite for full activation (PubMed:28264193, PubMed:35926713). mTORC2 catalyzes the phosphorylation of SGK1 at 'Ser-422' and of PRKCA on 'Ser-657' (PubMed:30837283, PubMed:35926713). The mTORC2 complex also phosphorylates various proteins involved in insulin signaling, such as FBXW8 and IGF2BP1 (By similarity). mTORC2 acts upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors (PubMed:15467718). mTORC2 promotes the serum-induced formation of stress-fibers or F-actin (PubMed:15467718). MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation (PubMed:15988011). Inhibits HRAS and KRAS independently of mTORC2 complex (PubMed:17303383, PubMed:34380736, PubMed:35522713). Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription (PubMed:17054722). Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex (PubMed:23727834)|In contrast to isoform 1, isoform 2 and isoform 6, isoform 4 is not a component of the a mTORC2 complex

Subcellular Localization：Cell membrane,Endoplasmic reticulum membrane,Early endosome membrane,Late endosome membrane,Lysosome membrane,Golgi apparatus membrane,Mitochondrion outer membrane,Cytoplasm, perinuclear region,Nucleus,Cell membrane,Nucleus,Cell membrane,Nucleus,Cell membrane,Cytoplasm, cytosol,Cytoplasm,Nucleus

Expression：
Tissue_Specificity: Ubiquitously expressed, with highest levels in heart and skeletal muscle

Isoforms & Post-Translational Modification：Q9BPZ7 has 6 isomers: Q9BPZ7-1: 59123 Da (predicted); Q9BPZ7-2: 54971 Da (predicted); Q9BPZ7-3: 53744 Da (predicted); Q9BPZ7-4: 37320 Da (predicted); Q9BPZ7-5: 36237 Da (predicted); Q9BPZ7-6: 41838 Da (predicted).
Phosphorylation at Ser-128 by PKC promotes relocalization to the perinuclear region, where the mTORC2 complex specifically mediates phosphorylation of SGK1 (By similarity)

Subunit：Component of the mechanistic target of rapamycin complex 2 (mTORC2), consisting in two heterotretramers composed of MTOR, MLST8, RICTOR and MAPKAP1/SIN1 (PubMed:16919458, PubMed:16962653, PubMed:28264193, PubMed:29424687, PubMed:29567957, PubMed:33378666, PubMed:34519268, PubMed:35926713)

Synonyms

MAPKAP 1 antibody; MAPKAP1 antibody; MEKK2 interacting protein 1 antibody; MGC2745 antibody; MIP 1 antibody; MIP1 antibody; Mitogen activated protein kinase associated protein 1 antibody; Mitogen-activated protein kinase 2-associated protein 1 antibody; mSIN1 antibody; OTTHUMP00000064207 antibody

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)