Copy Link Print Information

CD166 Antibody (YA3492)

(Synonyms: activated leukocyte cell adhesion molecule; ALCAM protein; ALCAM; CD 166; CD166 antigen; FLJ38514; MEMD; MGC71733; FLJ3851; AI853494; BEN; DM-GRASP; MGC27910; MuSC; SC1; CD166_HUMAN.)
Customer Review

Based on 1 Customer Validation

CD166 Antibody (YA3492) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD166.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, FC, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD166 Antibody (YA3492)
  • CD166 Antibody (YA3492) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:50 1:50-1:200 1:50-1:100 1:20-1:50

Product Details

Description

CD166 Antibody (YA3492) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD166.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 105 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 65 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human CD166.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD166 Antibody (YA3492)
    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling CD166 Antibody (HY-P81138, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CD166 cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. Mediates homotypic interactions with cells that express ALCAM. Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation. Required for normal hematopoietic stem cell engraftment in the bone marrow. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation. Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons; Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation[1][2][3][4][5][6][7][8][9].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell projection, axon; Cell projection, dendrite; Secreted

  • Expression


    Tissue_specificity:Detected on hematopoietic stem cells derived from umbilical cord blood (PubMed:24740813) . Detected on lymph vessel endothelial cells, skin and tonsil (PubMed:23169771) . Detected on peripheral blood monocytes (PubMed:15048703) . Detected on monocyte-derived dendritic cells (at protein level) (PubMed:16352806) . Detected at low levels in spleen, placenta, liver (PubMed:9502422) . Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells (PubMed:7760007) . Isoform 1 and isoform 3 are detected in vein and artery endothelial cells, astrocytes, keratinocytes and artery smooth muscle cells (PubMed:15496415) . Expressed by neurons in the brain. Restricted expression in tumor cell lines. Detected in highly metastasizing melanoma cell lines (PubMed:9502422)

    Induction:Up-regulated by TNF and IFNG (at protein level)

  • Isoforms & Post-Translational Modification

    Q13740 has 4 isomers: Q13740-1: 65102 Da (predicted); Q13740-2: 63665 Da (predicted); Q13740-3: 15109 Da (predicted); Q13740-4: 33878 Da (predicted).
    Glycosylated

  • Subunit

    Homodimer (PubMed:15048703, PubMed:16352806, PubMed:26146185, PubMed:7760007). Interacts (via extracellular domain) with CD6 (via extracellular domain) (PubMed:15048703, PubMed:16914752, PubMed:24945728, PubMed:26146185, PubMed:7760007, PubMed:8823162).

  • SwissProt ID

    Q13740

  • Gene ID
    214 [NCBI]
  • Synonyms

    activated leukocyte cell adhesion molecule; ALCAM protein; ALCAM; CD 166; CD166 antigen; FLJ38514; MEMD; MGC71733; FLJ3851; AI853494; BEN; DM-GRASP; MGC27910; MuSC; SC1; CD166_HUMAN.

[1]. Hassan NJ, et al. Frontline: Optimal T cell activation requires the engagement of CD6 and CD166. Eur J Immunol. 2004 Apr;34(4):930-40. [Content Brief]

[2]. Ikeda K, et al. Molecular isolation and characterization of a soluble isoform of activated leukocyte cell adhesion molecule that modulates endothelial cell function. J Biol Chem. 2004 Dec 31;279(53):55315-23. [Content Brief]

[3]. Zimmerman AW, et al. Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells. Blood. 2006 Apr 15;107(8):3212-20. [Content Brief]

[4]. Iolyeva M, et al. Novel role for ALCAM in lymphatic network formation and function. FASEB J. 2013 Mar;27(3):978-90. [Content Brief]

[5]. Escoda-Ferran C, et al. Modulation of CD6 function through interaction with Galectin-1 and -3. FEBS Lett. 2014 Aug 25;588(17):2805-13. [Content Brief]

[6]. Bowen MA, et al. Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand. J Exp Med. 1995 Jun 1;181(6):2213-20. [Content Brief]

[7]. Gimferrer I, et al. Relevance of CD6-mediated interactions in T cell activation and proliferation. J Immunol. 2004 Aug 15;173(4):2262-70. [Content Brief]

[8]. Xu Z, et al. ILT3.Fc-CD166 Interaction Induces Inactivation of p70 S6 Kinase and Inhibits Tumor Cell Growth. J Immunol. 2018 Feb 1;200(3):1207-1219. [Content Brief]

[9]. Chitteti BR, et al. CD166 regulates human and murine hematopoietic stem cells and the hematopoietic niche. Blood. 2014 Jul 24;124(4):519-29. [Content Brief]

View More

CD166 Antibody (YA3492) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling CD166 Antibody (HY-P81138, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD166 Antibody (YA3492) Product Image
    1/16
  • Experimental Validation Results for CD166 Antibody (YA3492)
  • CD166 Antibody (YA3492) Product Image
  • Experimental Validation Results for CD166 Antibody (YA3492)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling CD166 Antibody (HY-P81138, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD166 Antibody (YA3492)
  • CD166 Antibody (YA3492) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling CD166 Antibody (HY-P81138, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD166 Antibody (YA3492) Product Image FC