ATG3 Antibody (YA602)

Cat. No.: HY-P80556: Data Sheet COA
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ATG3 Antibody (YA602) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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ATG3 Antibody (YA602) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

ATG3 Antibody (YA602) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG3.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 36 kDa;

Observed band size: 40 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9NT62

Gene ID

64422 [NCBI]

Immunogen

Synthetic peptide corresponding to Human ATG3 aa2-20.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB ICC

Western blot analysis of extracts from Hela (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg), using ATG3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling ATG3 with ATG3 Antibody (HY-P80556) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATG3 Antibody (HY-P80556) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Hela cells labeling ATG3 with ATG3 Antibody (HY-P80556) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATG3 Antibody (HY-P80556)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：E2 conjugating enzyme that catalyzes the covalent conjugation of the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to the amino group of phosphatidylethanolamine (PE)-containing lipids in the membrane resulting in membrane-bound ATG8-like proteins which is one of the key steps in the development of autophagic isolation membranes during autophagosome formation (PubMed:24191030, PubMed:33446636, PubMed:37252361). Cycles back and forth between binding to ATG7 for loading with the ATG8-like proteins and binding to E3 enzyme, composed of ATG12, ATG5 and ATG16L1 to promote ATG8-like proteins lipidation (PubMed:11825910, PubMed:12207896, PubMed:12890687, PubMed:16704426, PubMed:24186333). Also plays a role as a membrane curvature sensor that facilitates LC3/GABARAP lipidation by sensing local membrane stress associated with lipid-packing defects as occurs with high molar proportions of conical lipids or strident membrane curvature (By similarity). Interacts with negatively-charged membranes promoting membrane tethering and enhancing LC3/GABARAP lipidation (PubMed:29142222). Also acts as an autocatalytic E2-like enzyme by catalyzing the conjugation of ATG12 to itself in an ATG7-dependent manner, this complex thus formed, plays a role in mitochondrial homeostasis but not in autophagy (By similarity). ATG12-ATG3 conjugation promotes late endosome to lysosome trafficking and basal autophagosome maturation via its interaction with PDCD6IP (By similarity). ATG12-ATG3 conjugate is also formed upon viccina virus infection, leading to the disruption the cellular autophagy which is not necessary for vaccinia survival and proliferation (By similarity). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (By similarity)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:It is widely expressed, with the highest expression levels in the heart, skeletal muscle, kidneys, liver, and placenta.

Isoforms & Post-Translational Modification：Q9NT62 has 2 isomers: Q9NT62-1: 35864 Da (predicted); Q9NT62-2: 35467 Da (predicted).
Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha (PubMed:22644571). CASP8 cleavage blocks survival-related autophagy and favors apoptosis (PubMed:22644571);Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in phosphatidylethanolamine-conjugation to ATG8-like proteins and autophagy

Subunit：Homdimer (PubMed:24191030). Interacts with ATG7; this interaction forms an E1-E2 complex that is essential for the transfer of GABARAP thioester from ATG7 to ATG3 and disrupts interaction with the E3 enzyme complex (PubMed:11825910, PubMed:22170151, PubMed:24186333, PubMed:26043688, PubMed:37252361). Interacts with ATG12; this interaction is ATG7-dependent, essential for phosphatidylethanolamine (PE)-conjugated ATG8-like proteins formation and also mediates the autoconjugation of ATG12 on ATG3 (PubMed:11825910, PubMed:12207896, PubMed:22170151, PubMed:24191030). Interacts with FNBP1L (PubMed:19342671). Interacts with the E3 enzyme complex composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa); this interaction disrupts interaction with ATG7 and promotes ATG8-like proteins lipidation (PubMed:24186333, PubMed:24191030, PubMed:37252361). Interacts with GABARAP and MAP1LC3A (PubMed:37252361). Interacts with the ATG12-ATG5 conjugate; this interaction inhibits ATG8-like proteins lipidation (PubMed:12207896). Interacts (ATG12-ATG3 conjugate form) with PDCD6IP (via the BRO1 domain); this interaction is bridged by ATG12 and promotes multiple PDCD6IP-mediated functions such as endolysosomal trafficking, macroautophagy and exosome biogenesis (By similarity)

RRID

AB_3102609

Database

Entrez Gene: 64422 Human ; 67841 Mouse ; 171415 Rat

SwissProt: Q9NT62 Human ; Q9CPX6 Mouse ; Q6AZ50 Rat

OMIM: 609606 Human

Research Field

Cell Biology

Synonyms

APG3; APG3L; PC3-96; APG3-LIKE

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

ATG3 Antibody (YA602) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ATG3 Antibody (YA602)

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ATG3 Antibody (YA602) Related Classifications

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