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LRRC8A Antibody (YA8953)

(Synonyms: KIAA1437, LRRC8, SWELL1, UNQ221/PRO247, LRRC8A, Volume-regulated anion channel subunit LRRC8A, Leucine-rich repeat-containing protein 8A, Swelling protein 1, HsLRRC8A)
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Based on 1 Customer Validation

LRRC8A Antibody (YA8953) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to LRRC8A.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IP, ELISA

  • Reactivity :

    human, mouse, rat

  • Formulation:

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for LRRC8A Antibody (YA8953)
  • LRRC8A Antibody (YA8953) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:100-1000 1-2μg per 100-500μg Total protein 1:30-3000

Product Details

Description

LRRC8A Antibody (YA8953) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to LRRC8A.

  • Host Mouse
  • Species Reactivity
    human, mouse, rat
  • Calculated Molecular Weight Predicted band size: 94 kDa
Species Reactivity Database

OMIM: 608360

Immunogen

A synthesized peptide derived from human LRRC8A.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at 2-8°C for 1 year, do not freeze.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LRRC8A Antibody (YA8953)
    Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2), Jurkat (lane 3), NIH/3T3 (lane 4), and Rat lung (lane 5) using LRRC8A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    LRRC8A is essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress. LRRC8A and LRRC8D are required for the uptake of the drug cisplatin. In complex with LRRC8C or LRRC8E, acts as a transporter of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol: mediates both import and export of 2'-3'-cGAMP, thereby promoting transfer of 2'-3'-cGAMP to bystander cells. In contrast, complexes containing LRRC8D inhibit transport of 2'-3'-cGAMP. Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition. Can form functional channels by itself (in vitro). Involved in B-cell development: required for the pro-B cell to pre-B cell transition. Also required for T-cell development (By similarity). Required for myoblast differentiation: VRAC activity promotes membrane hyperpolarization and regulates insulin-stimulated glucose metabolism and oxygen consumption (By similarity). Also acts as a regulator of glucose-sensing in pancreatic beta cells: VRAC currents, generated in response to hypotonicity- or glucose-induced beta cell swelling, depolarize cells, thereby causing electrical excitation, leading to increase glucose sensitivity and insulin secretion. Also plays a role in lysosome homeostasis by forming functional lysosomal VRAC channels in response to low cytoplasmic ionic strength condition: lysosomal VRAC channels are necessary for the formation of large lysosome-derived vacuoles, which store and then expel excess water to maintain cytosolic water homeostasis. Acts as a key factor in NLRP3 inflammasome activation by modulating itaconate efflux and mitochondria function[1][2][3][4][5][6][7][8][9][10][11][12][13].

  • Subcellular Localization

    Cell membrane; Lysosome membrane

  • Expression


    Tissue_Specificity: Expressed in brain, kidney, ovary, lung, liver, heart, and fetal brain and liver. Found at high levels in bone marrow; lower levels are detected in peripheral blood cells. Expressed on T-cells as well as on B-lineage cells.

  • Isoforms & Post-Translational Modification

    LRRC8A has an amino acid length of 810, molecular weight is 94199 Da.
    N-glycosylated

  • Subunit

    Heterohexamer; oligomerizes with other LRRC8 proteins (LRRC8B, LRRC8C, LRRC8D and/or LRRC8E) to form a heterohexamer.

  • SwissProt ID

    Q8IWT6

  • Gene ID
    56262 [NCBI]
  • Synonyms

    KIAA1437, LRRC8, SWELL1, UNQ221/PRO247, LRRC8A, Volume-regulated anion channel subunit LRRC8A, Leucine-rich repeat-containing protein 8A, Swelling protein 1, HsLRRC8A

[1]. Qiu Z, et al. SWELL1, a plasma membrane protein, is an essential component of volume-regulated anion channel. Cell. 2014 Apr 10;157(2):447-458. [Content Brief]

[2]. Voss FK, et al. Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC. Science. 2014 May 9;344(6184):634-8. [Content Brief]

[3]. Planells-Cases R, et al. Subunit composition of VRAC channels determines substrate specificity and cellular resistance to Pt-based anti-cancer drugs. EMBO J. 2015 Dec 14;34(24):2993-3008. [Content Brief]

[4]. Syeda R, et al. LRRC8 Proteins Form Volume-Regulated Anion Channels that Sense Ionic Strength. Cell. 2016 Jan 28;164(3):499-511. [Content Brief]

[5]. Lutter D, et al. Selective transport of neurotransmitters and modulators by distinct volume-regulated LRRC8 anion channels. J Cell Sci. 2017 Mar 15;130(6):1122-1133. [Content Brief]

[6]. Deneka D, et al. Structure of a volume-regulated anion channel of the LRRC8 family. Nature. 2018 Jun;558(7709):254-259. [Content Brief]

[7]. Kefauver JM, et al. Structure of the human volume regulated anion channel. Elife. 2018 Aug 10;7:. [Content Brief]

[8]. Lahey LJ, et al. LRRC8A:C/E Heteromeric Channels Are Ubiquitous Transporters of cGAMP. Mol Cell. 2020 Nov 19;80(4):578-591.e5. [Content Brief]

[9]. Sawada A, et al. A congenital mutation of the novel gene LRRC8 causes agammaglobulinemia in humans. J Clin Invest. 2003 Dec;112(11):1707-13. [Content Brief]

[10]. Kang C, et al. SWELL1 is a glucose sensor regulating β-cell excitability and systemic glycaemia. Nat Commun. 2018 Jan 25;9(1):367. [Content Brief]

[11]. Lenk GM, et al. CRISPR knockout screen implicates three genes in lysosome function. Sci Rep. 2019 Jul 3;9(1):9609. [Content Brief]

[12]. Li P, et al. LRRC8 family proteins within lysosomes regulate cellular osmoregulation and enhance cell survival to multiple physiological stresses. Proc Natl Acad Sci U S A. 2020 Nov 17;117(46):29155-29165. [Content Brief]

[13]. Zhang F, et al. NOX4 Regulates NLRP3 by Inhibiting the Ubiquitination of LRRC8A to Promote Ferroptosis in Nucleus Pulposus Cells. Inflammation. 2025 Oct;48(5):3111-3129. [Content Brief]

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LRRC8A Antibody (YA8953) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2), Jurkat (lane 3), NIH/3T3 (lane 4), and Rat lung (lane 5) using LRRC8A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • LRRC8A Antibody (YA8953) Product Image
    1/16
  • Experimental Validation Results for LRRC8A Antibody (YA8953)
  • LRRC8A Antibody (YA8953) Product Image
  • Experimental Validation Results for LRRC8A Antibody (YA8953)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2), Jurkat (lane 3), NIH/3T3 (lane 4), and Rat lung (lane 5) using LRRC8A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for LRRC8A Antibody (YA8953)
  • LRRC8A Antibody (YA8953) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2), Jurkat (lane 3), NIH/3T3 (lane 4), and Rat lung (lane 5) using LRRC8A antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • LRRC8A Antibody (YA8953) Product Image WB