ATG13 Antibody (YA1650)(PBS only)

Cat. No.: HY-P81905A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

ATG13 Antibody (YA1650) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG13.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ATG13 Antibody (YA1650) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG13.

Host

Rabbit

Molecular Weight

Predicted band size: 57 kDa;

Observed band size: 72 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O75143

Gene ID

9776 [NCBI]

Immunogen

Recombinant protein of human KIAA0652 aa169-418.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation

Subcellular Localization：Cytoplasm, cytosol; Preautophagosomal structure

Isoforms & Post-Translational Modification：O75143 has 5 isomers: O75143-1: 56572 Da (predicted); O75143-2: 52823 Da (predicted); O75143-3: 44487 Da (predicted); O75143-4: 44093 Da (predicted); O75143-5: 60155 Da (predicted).
Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria

Subunit：Part of a complex consisting of ATG13, ULK1 and RB1CC1 (PubMed:19211835, PubMed:19225151, PubMed:19597335, PubMed:24290141). Interacts with ATG101 (PubMed:19287211, PubMed:19597335, PubMed:26299944). Interacts with ULK1 (via C-terminus); this interaction is increased in the absence of TMEM39A (PubMed:18936157, PubMed:19287211, PubMed:21855797, PubMed:31806350). Interacts with ULK2 (via C-terminus) (PubMed:18936157, PubMed:19225151). Interacts (via the LIR motif) with GABARAP, GABARAPL, GABARAPL2 (PubMed:23043107). Interacts (via the LIR motif) with MAP1LC3A, MAP1LC3B and MAP1LC3C (PubMed:24290141). Interacts with TAB2 and TAB3 (PubMed:21976705). Interacts with C9orf72 (PubMed:27334615). Interacts with RB1CC1; this interaction is increased in the absence of TMEM39A (PubMed:31806350)

Research Field

Cell Biology

Synonyms

Autophagy-related protein 13

Documentation

User Guide for Antibodies (1077 KB)