CD146 Antibody (YA5482)

Cat. No.: HY-P85790: Ficha de datos User Guide for Antibodies
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CD146 Antibody (YA5482) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD146.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

CD146 Antibody (YA5482) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD146.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 72 kDa;

Observed band size: 110 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P43121

Gene ID

4162 [NCBI]

Immunogen

Synthesized peptide derived from human CD146 AA range: 500-600

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1000
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:1000-5000

Pureza	Protein G	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration

Subcellular Localization：Membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:This gene can be detected in endothelial cells of vascular tissues throughout the body. During embryonic development, it may appear on the surface of neural crest cells as they migrate. In normal adult tissues, this gene appears to be limited to vascular smooth muscle. This gene is associated with tumor progression and metastasis in human malignant melanoma. It is most strongly expressed in metastatic lesions and advanced primary tumors, while it is rarely detected in benign melanocytic nevi and thin-layered primary melanomas with a low probability of metastasis.

Isoforms & Post-Translational Modification：P43121 has 2 isomers: P43121-1: 71607 Da (predicted); P43121-2: 57605 Da (predicted).

Synonyms

Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD antigen CD146;

Documentación

User Guide for Antibodies (1077 KB)