GADD45A Antibody (YA1076)(PBS only)

Cat. No.: HY-P81331A: Data Sheet User Guide for Antibodies
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GADD45A Antibody (YA1076) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GADD45A.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

GADD45A Antibody (YA1076) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GADD45A.

Host

Mouse

Molecular Weight

Predicted band size: 18 kDa;

Observed band size: 22 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P24522

Gene ID

1647 [NCBI]

Immunogen

Purified recombinant human GADD45 α protein expressed in E.coli

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase

Subcellular Localization：Nucleus

Expression：
Induction:By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediated by some kinase (s) other than PKC

Isoforms & Post-Translational Modification：P24522 has 2 isomers: P24522-1: 18336 Da (predicted); P24522-2: 14622 Da (predicted).

Subunit：Interacts with MAPK14 (By similarity). Predominantly monomeric but also forms dimers and other oligomers as concentration increases. Interacts with GADD45GIP1. Interacts weakly with PCNA. Interacts with AURKA, likely to compete with dimerization

Research Field

Epigenetics and Nuclear Signaling

Synonyms

DDIT1; GADD45

Documentation

User Guide for Antibodies (1077 KB)